Anti-DEP1/PTPRJ Antibody Picoband®

About this product

Boster Bio Anti-DEP1/PTPRJ Antibody Picoband® catalog # A02319-2. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02319-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Receptor-type tyrosine-protein phosphatase eta is an enzyme that in humans is encoded by the PTPRJ gene. The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms

Synaptotagmin-1; Synaptotagmin I; SytI; p65; SYT1; SVP65; SYT

Gene Name

PTPRJ

Gene ID

5795

UniProt

Q12913

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human DEP1/PTPRJ recombinant protein (Position: D143-E891) .

Clonality

Polyclonal

Tissue Specificity

Expressed in melanocytes (PubMed:23999003) .

Applications

ELISA, Flow Cytometry, IF, IHC, WB

Field of Research

Neuroscience, Neurotransmission, Protein Trafficking, Secretory Vesicles, Signal Transduction, Vesicle Transport

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca (2+) -dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca (2+) -independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes (PubMed:23999003) .

References & Citations

1. Honda, H., Inazawa, J., Nishida, J., Yazaki, Y., Hirai, H. Molecular cloning, characterization, and chromosomal localization of a novel protein-tyrosine phosphatase, HPTP eta. Blood 84: 4186-4194, 1994. 2. Lesueur, F., Pharoah, P. D., Laing, S., Ahmed, S., Jordan, C., Smith, P. L., Luben, R., Wareham, N. J., Easton, D. F., Dunning, A. M., Ponder, B. A. J. Allelic association of the human homologue of the mouse modifier Ptprj with breast cancer. Hum. Molec. Genet. 14: 2349-2356, 2005. 3. Li, Y., Haarhuis, J. H. I., Sedeno Cacciatore, A., Oldenkamp, R., van Ruiten, M. S., Willems, L., Teunissen, H., Muir, K. W., de Wit, E., Rowland, B. D., Panne, D. The structural basis for cohesin-CTCF-anchored loops. Nature 578: 472-476, 2020.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

33927 MW

Observed Molecular Weight

240 kDa

Applications Notes

6

Gene Name Synonym

Synaptotagmin 1

Subcellular Location

Cytoplasmic vesicle, secretory vesicle membrane.

Protein Name

Adrenocorticotropic hormone receptor

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.