Anti-UBC12/UBE2M Antibody Picoband®

About this product

Boster Bio Anti-UBC12/UBE2M Antibody Picoband® catalog # A07478-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A07478-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

NEDD8-conjugating enzyme Ubc12 is a protein that in humans is encoded by the UBE2M gene. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.

Synonyms

BAG family molecular chaperone regulator 5; BAG-5; Bcl-2-associated athanogene 5; BAG5; KIAA0873

Gene Name

UBE2M

Gene ID

9040

UniProt

P61081

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human UBC12/UBE2M recombinant protein (Position: K25-Q154) .

Clonality

Polyclonal

Tissue Specificity

Expressed in all tissues examined with lower levels in brain and testis.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Interleukins

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Inhibits both auto-ubiquitination of PARK2 and ubiquitination of target proteins by PARK2 (By similarity) . May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding. .

References & Citations

1. Huang, D. T., Hunt, H. W., Zhuang, M., Ohi, M. D., Holton, J. M., Schulman, B. A. Basis for a ubiquitin-like protein thioester switch toggling E1-E2 affinity. Nature 445: 394-398, 2007. 2. Osaka, F., Kawasaki, H., Aida, N., Saeki, M., Chiba, T., Kawashima, S., Tanaka, K., Kato, S. A new NEDD8-ligating system for cullin-4A. Genes Dev. 12: 2263-2268, 1998. 3. Scott, D. C., Monda, J. K., Bennett, E. J., Harper, J. W., Schulman, B. A. N-terminal acetylation acts as an avidity enhancer within an interconnected multiprotein complex. Science 334: 674-678, 2011.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

51200 MW

Observed Molecular Weight

21 kDa

Applications Notes

6

Gene Name Synonym

BAG family molecular chaperone regulator 5

Subcellular Location

Perinuclear region.

Protein Name

BAG family molecular chaperone regulator 5

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.