Anti-Ubc13/UBE2N Antibody Picoband®

About this product

Boster Bio Anti-Ubc13/UBE2N Antibody Picoband® catalog # A02462-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02462-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ubiquitin-conjugating enzyme E2 N is a protein that in humans is encoded by the UBE2N gene. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.

Synonyms

Probable ATP-dependent RNA helicase DDX4; DEAD box protein 4; Vasa homolog; DDX4; VASA

Gene Name

UBE2N

Gene ID

7334

UniProt

P61088

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Ubc13/UBE2N recombinant protein (Position: Q13-I152) .

Clonality

Polyclonal

Tissue Specificity

Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Signal Transduction, Cell Biology, Cell Cycle, Stem Cells, Cell Differentiation, Cytoskeleton/ECM, Extracellular Matrix, Structures, ECM Proteins, Mesenchymal Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600) . Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity) . Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity) . Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle (By similarity) . Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs (By similarity) .

References & Citations

1. Chen, J., Hao, L., Li, C., Ye, B., Du, Y., Zhang, H., Long, B., Zhu, P., Liu, B., Yang, L., Li, P., Tian, Y., Fan, Z. The endoplasmic reticulum adaptor protein ERAdP initiates NK cell activation via the Ubc13-mediated NF-kappa-B pathway. J. Immun. 194: 1292-303, 2015. 2. Fukushima, T., Matsuzawa, S., Kress, C. L., Bruey, J. M., Krajewska, M., Lefebvre, S., Zapata, J. M., Ronai, Z., Reed, J. C. Ubiquitin-conjugating enzyme Ubc13 is a critical component of TNF receptor-associated factor (TRAF) -mediated inflammatory responses. Proc. Nat. Acad. Sci. 104: 6371-6376, 2007. 3. Gross, M. B. Personal Communication. Baltimore, Md. 7/25/2014.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

38779 MW

Observed Molecular Weight

17 kDa

Applications Notes

6

Gene Name Synonym

DEAD-box helicase 4

Subcellular Location

Secreted, extracellular space, extracellular matrix.

Protein Name

BAG family molecular chaperone regulator 1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.