Anti-FFR/VPS51 Antibody Picoband®

About this product

Boster Bio Anti-FFR/VPS51 Antibody Picoband® catalog # A08518-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A08518-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a member of the vacuolar protein sorting-associated protein 51 family. The encoded protein is a component of the Golgi-associated retrograde protein complex which acts as a tethering factor for carriers in retrograde transport from the early and late endosomes to the trans-Golgi network. Alternative splicing results in multiple transcript variants.

Synonyms

PHD finger protein 21A; BHC80a; BRAF35-HDAC complex protein BHC80; PHF21A; BHC80; KIAA1696; BM-006

Gene Name

VPS51

Gene ID

738

UniProt

Q9UID3

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human FFR/VPS51 recombinant protein (Position: K38-R781) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed in brain. Expressed at much lower level in other tissues.

Applications

WB, Flow Cytometry, ELISA

Field of Research

Cancer, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it may act as a scaffold. Inhibits KDM1A-mediated demethylation of 'Lys-4' of histone H3 in vitro, suggesting a role in demethylation regulation.

References & Citations

1. Gershlick, D. C., Ishida, M., Jones, J. R., Bellomo, A., Bonifacino, J. S., Everman, D. B. A neurodevelopmental disorder caused by mutations in the VPS51 subunit of the GARP and EARP complexes. Hum. Molec. Genet. 28: 1548-1560, 2019. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 4/14/2014. 3. Perez-Victoria, F. J., Schindler, C., Magadan, J. G., Mardones, G. A., Delevoye, C., Romao, M., Raposo, G., Bonifacino, J. S. Ang2/fat-free is a conserved subunit of the Golgi-associated retrograde protein complex. Molec. Biol. Cell 21: 3386-3395, 2010.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

86 kDa

Applications Notes

6

Gene Name Synonym

PHD finger protein 21A

Subcellular Location

Nucleus.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.