Anti-DOCK180/DOCK1 Antibody Picoband®

About this product

Boster Bio Anti-DOCK180/DOCK1 Antibody Picoband® catalog # A03440-2. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03440-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Dock180, (Dedicator of cytokinesis) also known as DOCK1, is a large (~180 kDa) protein involved in intracellular signalling networks. This gene encodes a member of the dedicator of cytokinesis protein family. Dedicator of cytokinesis proteins act as guanine nucleotide exchange factors for small Rho family G proteins. The encoded protein regulates the small GTPase Rac, thereby influencing several biological processes, including phagocytosis and cell migration. Overexpression of this gene has also been associated with certain cancers. Alternative splicing results in multiple transcript variants.

Synonyms

Tumor necrosis factor-inducible gene 6 protein; Hyaluronate-binding protein; TNF-stimulated gene 6 protein; TSG-6; Tumor necrosis factor alpha-induced protein 6; TNF alpha-induced protein 6; TNFAIP6; TSG6

Gene Name

DOCK1

Gene ID

1793

UniProt

Q14185

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human DOCK180/DOCK1 recombinant protein (Position: S100-T1732) .

Clonality

Polyclonal

Tissue Specificity

Found in the synovial fluid of patients with rheumatoid arthritis. .

Applications

ELISA, Flow Cytometry, IF, IHC, WB

Field of Research

Cancer, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Immunology, Innate Immunity, Invasion/Microenvironment, Macrophage/Inflammation, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Possibly involved in cell-cell and cell-matrix interactions during inflammation and tumorigenesis.

References & Citations

1. Cote, J.-F., Vuori, K. Identification of an evolutionarily conserved superfamily of DOCK180-related proteins with guanine nucleotide exchange activity. J. Cell Sci. 115: 4901-4913, 2002. 2. Gross, M. B. Personal Communication. Baltimore, Md. 8/31/2016. 3. Hasegawa, H., Kiyokawa, E., Tanaka, S., Nagashima, K., Gotoh, N., Shibuya, M., Kurata, T., Matsuda, M. DOCK180, a major CRK-binding protein, alters cell morphology upon translocation to the membrane. Molec. Cell Biol. 16: 1770-176, 1996.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

31203 MW

Observed Molecular Weight

215 kDa

Applications Notes

6

Gene Name Synonym

Tumor necrosis factor-inducible gene 6 protein

Subcellular Location

Late endosome membrane. Peripheral membrane protein. Prevacuolar compartment membrane.

Protein Name

Tumor necrosis factor-inducible gene 6 protein

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.