Anti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody Picoband®

About this product

Boster Bio Anti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody Picoband® catalog # A06668-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A06668-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tripeptidyl-peptidase 2 is an enzyme that in humans is encoded by the TPP2 gene. This gene encodes a mammalian peptidase that, at neutral pH, removes tripeptides from the N terminus of longer peptides. The protein has a specialized function that is essential for some MHC class I antigen presentation. The protein is a high molecular mass serine exopeptidase; the amino acid sequence surrounding the serine residue at the active site is similar to the peptidases of the subtilisin class rather than the trypsin class.

Synonyms

Ubiquitin carboxyl-terminal hydrolase 21; Deubiquitinating enzyme 21; Ubiquitin thioesterase 21; Ubiquitin-specific-processing protease 21; USP21; USP23; PP1490

Gene Name

TPP2

Gene ID

7174

UniProt

P29144

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Tripeptidyl peptidase II/TPPII/TPP2 recombinant protein (Position: E32-K1001) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Cofactors, Vitamins/Minerals, Metabolism, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Pathways and Processes, Signal Transduction, Vitamins/Minerals

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination. Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates. Deubiquitinates BAZ2A/TIP5 leading to its stabilization.

References & Citations

1. Atallah, I., Quinodoz, M., Campos-Xavier, B., Peter, V. G., Fouriki, A., Bonvin, C., Bottani, A., Kumps, C., Angelini, F., Bellutti Enders, F., Christen-Zaech, S., Rizzi, M., and 11 others. Immune deficiency, autoimmune disease and intellectual disability: a pleiotropic disorder caused by biallelic variants in the TPP2 gene. Clin. Genet. 99: 780-788, 2021. 2. Balow, R.-M., Ragnarsson, U., Zetterqvist, O. Tripeptidyl aminopeptidase in the extralysosomal fraction of rat liver. J. Biol. Chem. 258: 11622-11628, 1983. 3. Bermingham, N. A., McKay, T., Hoyle, J., Hernandez, D., Martin, J. E., Fisher, E. M. C. The gene encoding tripeptidyl peptidase II maps to chromosome 1 in the mouse. Mammalian Genome 7: 390 only, 1996.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

104553 MW

Observed Molecular Weight

138 kDa

Applications Notes

6

Gene Name Synonym

Ubiquitin specific peptidase 21

Subcellular Location

Nucleus. Cytoplasm.

Protein Name

AP-2 complex subunit beta

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.