Anti-MG53/TRIM72 Antibody Picoband®

About this product

Boster Bio Anti-MG53/TRIM72 Antibody Picoband® catalog # A06982-2. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A06982-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Enables identical protein binding activity. Predicted to be involved in several processes, including cellular protein metabolic process; plasma membrane repair; and protein homooligomerization. Predicted to act upstream of or within negative regulation of insulin receptor signaling pathway; negative regulation of insulin-like growth factor receptor signaling pathway; and negative regulation of myotube differentiation. Predicted to be located in cytoplasmic vesicle membrane. Predicted to be active in cytoplasm and sarcolemma.

Synonyms

Aflatoxin B1 aldehyde reductase member 2; AFB1 aldehyde reductase 1; AFB1-AR 1; Aldoketoreductase 7; Succinic semialdehyde reductase; SSA reductase; AKR7A2; AFAR; AFAR1; AKR7

Gene Name

TRIM72

Gene ID

493829

UniProt

Q6ZMU5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MG53/TRIM72 recombinant protein (Position: E28-A477) .

Clonality

Polyclonal

Tissue Specificity

Expressed in basement membranes of lung and kidney. Muscle- and neuron-specific isoforms are found. Isoforms (y+) with the 4 AA insert and (z+8) isoforms with the 8 AA insert are all neuron-specific. Isoforms (z+11) are found in both neuronal and non-neuronal tissues.

Applications

ELISA, Flow Cytometry, IF, IHC, WB

Field of Research

Epigenetics and Nuclear Signaling, Pol II Transcription, Polymerase Associated Factors, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB) . Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2-nitrobenzaldehyde and pyridine-2-aldehyde (in vitro) . Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro) . Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.

References & Citations

1. Cai, C., Masumiya, H., Weisleder, N., Matsuda, N., Nishi, M., Hwang, M., Ko, J.-K., Lin, P., Thornton, A., Zhao, X., Pan, Z., Komazaki, S., Brotto, M., Takeshima, H., Ma, J. MG53 nucleates assembly of cell membrane repair machinery. Nature Cell Biol. 11: 56-64, 2009. 2. Song, R., Peng, W., Zhang, Y., Lv, F., Wu, H.-K., Guo, J., Cao, Y., Pi, Y., Zhang, X., Jin, L., Zhang, M., Jiang, P., Liu, F., Meng, S., Zhang, X., Jiang, P., Cao, C.-M., Xiao, R.-P. Central role of E3 ubiquitin ligase MG53 in insulin resistance and metabolic disorders. Nature 494: 375-379, 2013. 3. Zhu, H., Lin, P., De, G., Choi, K., Takeshima, H., Weisleder, N., Ma, J. Polymerase transcriptase release factor (PTRF) anchors MG53 protein to cell injury site for initiation of membrane repair. J. Biol. Chem. 286: 12820-12824, 2011.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

87148 MW

Observed Molecular Weight

55 kDa

Applications Notes

6

Gene Name Synonym

Aldo-keto reductase family 7 member A2

Subcellular Location

Golgi apparatus. Cytoplasm.

Protein Name

ADAM metallopeptidase domain 28

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.