Anti-XRCC4 Antibody Picoband®

About this product

Boster Bio Anti-XRCC4 Antibody Picoband® catalog # A00787-2. Tested in ELISA, Flow Cytometry, ICC, IHC, IF, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00787-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

DNA repair protein XRCC4, also known as X-ray repair cross-complementing protein 4 or XRCC4, is a protein that in humans is encoded by the XRCC4 gene. In addition to humans, the XRCC4 protein is also expressed in many other metazoans, fungi and in plants. The X-ray repair cross-complementing protein 4 is one of several coreproteins involved in the non-homologous end joining (NHEJ) pathway to repair DNA double strand breaks (DSBs) . Since XRCC4 is the key protein that enables interaction of LigIV to damaged DNA and therefore ligation of the ends, mutations in the XRCC4 gene were found to cause embryonic lethality in mice and developmental inhibition and immunodeficiency in humans. Furthermore, certain mutations in the XRCC4 gene are associated with an increased risk of cancer.

Synonyms

Estrogen receptor beta; ER-beta; Nuclear receptor subfamily 3 group A member 2; ESR2; ESTRB, NR3A2

Gene Name

XRCC4

Gene ID

7518

UniProt

Q13426

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human XRCC4 recombinant protein (Position: M1-D255) .

Clonality

Polyclonal

Tissue Specificity

Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Cancer,2339, Diabetes, Domain Families, Endocrine System, Epigenetics and Nuclear Signaling, Metabolism, Neuroscience, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling Pathways, Obesity, Signal Transduction, Signaling Pathway, Transcription, Tumor Biomarkers, Zinc Finger

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560) . Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. .

References & Citations

1. Shao N, Jiang WY, Qiao D, Zhang SG, Wu Y, Zhang XX, Hua LX, Ding Y, Feng NH (2012) . An updated meta-analysis of XRCC4 polymorphisms and cancer risk based on 31 case-control studies. Cancer Biomark 12 (1) : 37–47. 2. West CE, Waterworth WM, Jiang Q, Bray CM (October 2000) . Arabidopsis DNA ligase IV is induced by gamma-irradiation and interacts with an Arabidopsis homologue of the double strand break repair protein XRCC4.Plant J. 24 (1) : 67–78.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

59216 MW

Observed Molecular Weight

55 kDa

Applications Notes

6

Gene Name Synonym

Estrogen receptor beta

Subcellular Location

Nucleus .

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.