Anti-TGF beta Receptor III/TGFBR3 Antibody Picoband®

About this product

Boster Bio Anti-TGF beta Receptor III/TGFBR3 Antibody Picoband® catalog # A02765-5. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02765-5

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Betaglycan also known as Transforming growth factor beta receptor III (TGFBR3), is a cell-surface chondroitin sulfate / heparan sulfate proteoglycan >300 kDa in molecular weight. This locus encodes the transforming growth factor (TGF) -beta type III receptor. The encoded receptor is a membrane proteoglycan that often functions as a co-receptor with other TGF-beta receptor superfamily members. Ectodomain shedding produces soluble TGFBR3, which may inhibit TGFB signaling. Decreased expression of this receptor has been observed in various cancers. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Synonyms

CD3 antigen, epsilon polypeptide; CD3e molecule; Cd3e; Cd3e_predicted; rCG_58471

Gene Name

TGFBR3

Gene ID

7049

UniProt

Q03167

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human TGF beta Receptor III/TGFBR3 recombinant protein (Position: H37-R649) .

Clonality

Polyclonal

Applications

WB, Flow Cytometry, ELISA

Field of Research

Cardiovascular, Chemokines, Immunology, Innate Immunity, Platelets

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed:19956738, PubMed:24899501) . Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region.

References & Citations

1. Brown, C. B., Boyer, A. S., Runyan, R. B., Barnett, J. V. Requirement of type III TGF-beta receptor for endocardial cell transformation in the heart. Science 283: 2080-2082, 1999. 2. Chen, W., Kirkbride, K. C., How, T., Nelson, C. D., Mo, J., Frederick, J. P., Wang, X.-F., Lefkowitz, R. J., Blobe, G. C. Beta-arrestin 2 mediates endocytosis of type III TGF-beta receptor and down-regulation of its signaling. Science 301: 1394-1397, 2003. 3. Dong, M., How, T., Kirkbride, K. C., Gordon, K. J., Lee, J. D., Hempel, N., Kelly, P., Moeller, B. J., Marks, J. R., Blobe, G. C. The type III TGF-beta receptor suppresses breast cancer progression. J. Clin. Invest. 117: 206-217, 2007.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

12252 MW

Observed Molecular Weight

150 kDa

Applications Notes

6

Gene Name Synonym

CD3e molecule

Subcellular Location

Alpha-beta T cell receptor complex; external side of plasma membrane; immunological synapse; cell body; cell-cell junction; dendritic spine; integral component of membrane

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.