Anti-ACK1/TNK2 Antibody Picoband®

About this product

Boster Bio Anti-ACK1/TNK2 Antibody Picoband® catalog # A02334-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02334-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Activated CDC42 kinase 1, also known as ACK1, is an enzyme that in humans is encoded by the TNK2 gene. This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP) -stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is ly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.

Synonyms

Acid-sensing ion channel 1; ASIC1; Amiloride-sensitive cation channel 2, neuronal; Brain sodium channel 2; BNaC2; ASIC1; ACCN2, BNAC2

Gene Name

TNK2

Gene ID

10188

UniProt

Q07912

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human TNK2/ACK1 recombinant protein (Position: M491-R1038) .

Clonality

Polyclonal

Tissue Specificity

Expressed in most or all neurons.

Applications

WB, Flow Cytometry, ELISA

Field of Research

Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Isoform 2 and isoform 3 function as proton-gated sodium channels; they are activated by a drop of the extracellular pH and then become rapidly desensitized. The channel generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Isoform 2 can also transport potassium, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 can also transport calcium ions. Mediates glutamate-independent Ca2+entry into neurons upon acidosis. This Ca2+overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca2+concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.

References & Citations

1. Hitomi, Y., Heinzen, E. L., Donatello, S., Dahl, H.-H., Damiano, J. A., McMahon, J. M., Berkovic, S. F., Scheffer, I. E., Legros, B., Rai, M., Weckhuysen, S., Suls, A., De Jonghe, P., Pandolfo, M., Goldstein, D. B., Van Bogaert, P., Depondt, C. Mutations in TNK2 in severe autosomal recessive infantile onset epilepsy. Ann. Neurol. 74: 496-501, 2013. 2. La Torre, A., del Rio, J. A., Soriano, E., Urena, J. M. Expression pattern of ACK1 tyrosine kinase during brain development in the mouse. Gene Expr. Patterns 6: 886-892, 2006. 3. Manser, E., Leung, T., Salihuddin, H., Tan, L., Lim, L. A non-receptor tyrosine kinase that inhibits the GTPase activity of p21cdc42. Nature 363: 364-367, 1993.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

115 kDa

Applications Notes

6

Gene Name Synonym

Acid sensing ion channel subunit 1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.