Anti-Milk Fat Globule 1/MFGE8 Antibody Picoband®

About this product

Boster Bio Anti-Milk Fat Globule 1/MFGE8 Antibody Picoband® catalog # A02518-2. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02518-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, Collins et al. (1997) mapped the MFGE8 gene to chromosome 15q25. Hanayama et al. (2002) found that MFGE8 is a factor that links apoptotic cells to phagocytes. MFGE8 specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. Bu et al. (2007) showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE) -dependent mechanism.

Synonyms

Tumor necrosis factor receptor superfamily member 4; MRC OX40; OX40 antigen; OX40L receptor; CD134; Tnfrsf4; Ox40; Txgp1l

Gene Name

MFGE8

Gene ID

4240

UniProt

Q08431

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Milk Fat Globule 1/MFGE8, which shares 70.6% amino acid (aa) sequence identity with mouse and rat MFGE8.

Clonality

Polyclonal

Applications

WB, IHC

Field of Research

Adaptive Immunity, Autoimmune, B Cells, Cytokines, Hematopoietic Progenitors, Immune System Diseases, Immunology, Innate Immunity, Lymphoid, Stem Cells, T Cells, T Lymphocytic Lineage, TNF Superfamily

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity (By similarity) . .

References & Citations

1. Bu, H.-F., Zuo, X.-L., Wang, X., Ensslin, M. A., Koti, V., Hsueh, W., Raymond, A. S., Shur, B. D., Tan, X.-D. Milk fat globule-EGF factor 8/lactadherin plays a crucial role in maintenance and repair of murine intestinal epithelium. J. Clin. Invest. 117: 3673-3683, 2007. 2. Collins, C., Nehlin, J. O., Stubbs, J. D., Kowbel, D., Kuo, W.-L., Parry, G. Mapping of a newly discovered human gene homologous to the apoptosis associated-murine mammary protein, MFG-E8, to chromosome 15q25. Genomics 39: 117-118, 1997. 3. Hanayama, R., Tanaka, M., Miwa, K., Shinohara, A., Iwamatsu, A., Nagata, S. Identification of a factor that links apoptotic cells to phagocytes. Nature 417: 182-187, 2002.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

43 kDa

Gene Name Synonym

TNF receptor superfamily member 4

Subcellular Location

Membrane; Single-pass type I membrane protein.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.