Anti-OAT3/SLC22A8 Antibody Picoband®

About this product

Boster Bio Anti-OAT3/SLC22A8 Antibody Picoband® catalog # A04087-2. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A04087-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Solute carrier family 22 member 8, or organic anion transporter 3 (OAT3), is a protein that in humans is encoded by the SLC22A8 gene. This gene encodes a protein involved in the sodium-independent transport and excretion of organic anions, some of which are potentially toxic. The encoded protein is an integral membrane protein and appears to be localized to the basolateral membrane of the kidney. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.

Synonyms

Muscarinic acetylcholine receptor M1; CHRM1

Gene Name

SLC22A8

Gene ID

9376

UniProt

Q8TCC7

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human OAT3/SLC22A8 recombinant protein (Position: M1-Q470) .

Clonality

Polyclonal

Tissue Specificity

Sperm. Mainly localized in the tail and in the postacrosomal region but is also found in the midpiece and basal region in a small percentage of sperm cells. Reduced levels found in the sperms of asthenozoospermia and leukocytospermia patients (at protein level) . Spleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes.

Applications

ELISA, Flow Cytometry, IF, IHC, WB

Field of Research

Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

References & Citations

1. Race, J. E., Grassl, S. M., Williams, W. J., Holtzman, E. J. Molecular cloning and characterization of two novel human renal organic anion transporters (hOAT1 and hOAT3) . Biochem. Biophys. Res. Commun. 255: 508-514, 1999. 2. Sun, W., Wu, R. R., van Poelje, P. D., Erion, M. D. Isolation of a family of organic anion transporters from human liver and kidney. Biochem. Biophys. Res. Commun. 283: 417-422, 2001.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

211344 MW

Observed Molecular Weight

62 kDa

Applications Notes

6

Gene Name Synonym

Cholinergic receptor muscarinic 1

Subcellular Location

Cell membrane. Multi-pass membrane protein. Postsynaptic cell membrane. Multi-pass membrane protein.

Protein Name

Chromodomain-helicase-DNA-binding protein 2

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.