Anti-TWEAK/Tnfsf12 Antibody Picoband®

About this product

Boster Bio Anti-TWEAK/Tnfsf12 Antibody Picoband® catalog # A02009-3. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02009-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tumor necrosis factor ligand superfamily member 12 also known as TNF-related weak inducer of apoptosis (TWEAK) is a protein that in humans is encoded by the TNFSF12 gene. Predicted to enable receptor ligand activity. Acts upstream of or within extrinsic apoptotic signaling pathway. Located in extracellular region. Is expressed in Meckel's cartilage; hip; skeleton; and superior cervical ganglion. Orthologous to several human genes including TNFSF12-TNFSF13 (TNFSF12-TNFSF13 readthrough) .

Synonyms

Tumor necrosis factor ligand superfamily member 12; APO3 ligand; TNF-related weak inducer of apoptosis; TWEAK; Tumor necrosis factor ligand superfamily member 12, membrane form; Tumor necrosis factor ligand superfamily member 12, secreted form; TNFSF12; APO3L, DR3LG; UNQ181/PRO207

Gene Name

Tnfsf12

Gene ID

21944

UniProt

O54907

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived mouse TWEAK/Tnfsf12 recombinant protein (Position: S46-H249) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain.

Applications

WB, ELISA

Field of Research

Apoptosis, Cancer, Cell Biology, Cell Death, Growth Factors, Invasion/Microenvironment

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion.

References & Citations

1. Chicheportiche, Y., Bourdon, P. R., Xu, H., Hsu, Y.-M., Scott, H., Hession, C., Garcia, I., Browning, J. L. TWEAK, a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. J. Biol. Chem. 272: 32401-32410, 1997. 2. Jain, M., Jakubowski, A., Cui, L., Shi, J., Su, L., Bauer, M., Guan, J., Lim, C. C., Naito, Y., Thompson, J. S., Sam, F., Ambrose, C., and 9 others. A novel role for tumor necrosis factor-like weak inducer of apoptosis (TWEAK) in the development of cardiac dysfunction and failure. Circulation 119: 2058-2068, 2009. 3. Kaptein, A., Jansen, M., Dilaver, G., Kitson, J., Dash, L., Wang, E., Owen, M. J., Bodmer, J.-L., Tschopp, J., Farrow, S. N. Studies on the interaction between TWEAK and the death receptor WSL-1/TRAMP (DR3) . FEBS Lett. 485: 135-141, 2000.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

62616 MW

Observed Molecular Weight

27 kDa

Gene Name Synonym

TNF superfamily member 12

Subcellular Location

Cell membrane.

Protein Name

Bifunctional epoxide hydrolase 2

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.