Anti-Pin1 Antibody Picoband® (monoclonal, 5E5)

About this product

Boster Bio Anti-Pin1 Antibody Picoband® (monoclonal, 5E5) catalog # M00467-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M00467-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, also called DOD, is an enzyme that in humans is encoded by the PIN1 gene. It is mapped to 19p13.2. The enzyme binds to a subset of proteins and thus plays a role as a post phospho PIN1rol in regulating protein function. Studies have shown that the deregulation of PIN1 may play a pivotal role in various diseases. Notably, the up-regulation of PIN1 may be implicated in certain cancers, and the down-regulation of Pin1 may be implicated in Alzheimer's disease. Inhibitors of PIN1 may have therapeutic implications for cancer and immune disorders. PIN1 activity regulates the outcome of proline-ed kinase (e.g. MAPK, CDK or GSK3) signalling and consequently regulates cell proliferation (in part through control of cyclin D1 levels and stability) and cell survival. PIN1 also has an essential role in maintaining cell proliferation and regulating cyclin D1 function.

Synonyms

Catechol O-methyltransferase; COMT

Gene Name

PIN1

Gene ID

5300

UniProt

Q13526

Host

Mouse

Reactivity

Human, Monkey, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Pin1 recombinant protein (Position: M1-E163) . Human Pin1 shares 95% amino acid (aa) sequence identity with mouse Pin1.

Clonality

Monoclonal

Clone

Clone: 5E5

Tissue Specificity

Brain, liver, placenta, lymphocytes and erythrocytes.

Applications

WB, ICC, IF, Flow Cytometry

Field of Research

Cell Adhesion, Cell Type Markers, Cytoskeleton/ECM, Hematopoietic Progenitors, Immunology, Integrins, Myeloid, Signal Transduction, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.

References & Citations

1. Liou, Y.-C., Ryo, A., Huang, H.-K., Lu, P.-J., Bronson, R., Fujimori, F., Uchida, T., Hunter, T., Lu, K. P. Loss of Pin1 function in the mouse causes phenotypes resembling cyclin D1-null phenotypes. Proc. Nat. Acad. Sci. 99: 1335-1340, 2002. 2. Lu, KP; Finn G; Lee TH; Nicholson LK (Oct 2007) . Prolyl cis-trans isomerization as a molecular timer.Nat. Chem. Biol. (United States) 3 (10) : 619–29.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

18 kDa

Applications Notes

6

Gene Name Synonym

Catechol-O-methyltransferase

Subcellular Location

Cytoplasm. Cell membrane. Single-pass type II membrane protein. Extracellular side.

Protein Name

Integrin beta-2

Isotype

IgG2b

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.