Anti-IFI16 Antibody Picoband® (monoclonal, 2I3D7)

About this product

Boster Bio Anti-IFI16 Antibody Picoband® (monoclonal, 2I3D7) catalog # M00848. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M00848

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Gamma-interferon-inducible protein Ifi-16 (Ifi-16) also known as interferon-inducible myeloid differentiation transcriptional activator is a protein that in humans is encoded by the IFI16 gene. This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Synonyms

Dihydrofolate reductase; DHFR

Gene Name

IFI16

Gene ID

3428

UniProt

Q16666

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human IFI16 recombinant protein (Position: E183-K743) .

Clonality

Monoclonal

Clone

Clone: 2I3D7

Tissue Specificity

Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain.

Applications

WB, ICC, IF, Flow Cytometry

Field of Research

Amino Acid Metabolism, Amino Acids, Cancer, Cell Biology, Metabolic Signaling Pathways, Metabolism, Nucleotide Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.

References & Citations

1. Choubey, D., Snoddy, J., Chaturvedi, V., Toniato, E., Opdenakker, G., Thakur, A., Samanta, H., Engel, D. A., Lengyel, P. Interferons as gene activators: indications for repeated gene duplication during the evolution of a cluster of interferon-activatable genes on murine chromosome 1. J. Biol. Chem. 264: 17182-17189, 1989. 2. Kerur, N., Veettil, M. V., Sharma-Walia, N., Bottero, V., Sadagopan, S., Otageri, P., Chandran, B. IFI16 acts as a nuclear pathogen sensor to induce the inflammasome in response to Kaposi sarcoma-associated herpesvirus infection. Cell Host Microbe 9: 363-375, 2011. 3. Kingsmore, S. F., Snoddy, J., Choubey, D., Lengyel, P., Seldin, M. F. Physical mapping of a family of interferon-activated genes, serum amyloid P-component, and alpha-spectrin on mouse chromosome 1. Immunogenetics 30: 169-174, 1989.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

98 kDa

Applications Notes

6

Gene Name Synonym

Dihydrofolate reductase

Subcellular Location

Mitochondrion. Cytoplasm

Protein Name

Zeta chain of T cell receptor associated protein kinase 70

Isotype

IgG2b

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.