Anti-EIF2AK1 Antibody Picoband®

About this product

Boster Bio Anti-EIF2AK1 Antibody Picoband® catalog # A05465-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05465-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Eukaryotic translation initiation factor 2-alpha kinase 1 is an enzyme that in humans is encoded by the EIF2AK1 gene. The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

Prion-like protein doppel; PrPLP; Prion protein 2; PRND; DPL; UNQ1830/PRO3443

Gene Name

EIF2AK1

Gene ID

27102

UniProt

Q9BQI3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human EIF2AK1 recombinant protein (Position: E37-D508) .

Clonality

Polyclonal

Tissue Specificity

Expressed in testis, in Sertoli cells, ejaculated spermatozoa and in seminal fluid (at protein level) .

Applications

WB, IHC, ICC, IF, ELISA

Field of Research

Neurodegenerative Disease, Neurology Process, Neuroscience

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Required for normal acrosome reaction and for normal male fertility (By similarity) . Can bind Cu (2+) (PubMed:15218028, PubMed:20411530) .

References & Citations

1. Abdel-Nour, M., Carneiro, L. A. M., Downey, J., Tsalikis, J., Outlioua, A., Prescott, D., Da Costa, L. S., Hovingh, E. S., Farahvash, A., Gaudet, R. G., Molinaro, R., van Dalen, R., and 11 others. The heme-regulated inhibitor is a cytosolic sensor of protein misfolding that controls innate immune signaling. Science 365: eaaw4144, 2019. Note: Electronic Article. 2. Acharya, P., Chen, J.-J., Correia, M. A. Hepatic heme-regulated inhibitor (HRI) eukaryotic initiation factor 2-alpha kinase: a protagonist of heme-mediated translational control of CYP2B enzymes and a modulator of basal endoplasmic reticulum stress tone. Molec. Pharm. 77: 575-592, 2010. Note: Retraction: Molec. Pharm. 100: 95 only, 2021. 3. Chen, J.-J., London, I. M. Regulation of protein synthesis by heme-regulated eIF-2-alpha kinase. Trends Biochem. Sci. 20: 105-108, 1995.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

39411 MW

Observed Molecular Weight

71 kDa

Gene Name Synonym

Prion like protein doppel

Subcellular Location

Cell membrane.

Protein Name

Zinc finger protein Helios

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.