Anti-SNRPD2 Antibody Picoband®

About this product

Boster Bio Anti-SNRPD2 Antibody Picoband® catalog # A12026-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A12026-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Small nuclear ribonucleoprotein Sm D2 is a protein that in humans is encoded by the SNRPD2 gene. The protein encoded by this gene belongs to the small nuclear ribonucleoprotein core protein family. It is required for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms

Negative elongation factor B; NELF-B; Cofactor of BRCA1; NELFB; COBRA1; KIAA1182

Gene Name

SNRPD2

Gene ID

6633

UniProt

P62316

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SNRPD2 recombinant protein (Position: M1-K118) .

Clonality

Polyclonal

Tissue Specificity

Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Epigenetics and Nuclear Signaling, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. May be able to induce chromatin unfolding.

References & Citations

1. Lehmeier, T., Raker, V., Hermann, H., Luhrmann, R. cDNA cloning of the Sm proteins D2 and D3 from human small nuclear ribonucleoproteins: evidence for a D1-D2 interaction. Proc. Nat. Acad. Sci. 91: 12317-12321, 1994. 2. Rokeach, L. A., Haselby, J. A., Hoch, S. O. Molecular cloning of a cDNA encoding the human Sm-D antigen. Proc. Nat. Acad. Sci. 85: 4832-4836, 1988.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

16 kDa

Applications Notes

6

Gene Name Synonym

Negative elongation factor complex member B

Subcellular Location

Nucleus.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.