Anti-SPATA5 Antibody Picoband®

About this product

Boster Bio Anti-SPATA5 Antibody Picoband® catalog # A06364-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A06364-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Spermatogenesis-associated protein 5 is a protein that in humans is encoded by the SPATA5 gene. This gene encodes a member of the ATPase associated with diverse activities family, whose members are defined by a highly conserved ATPase domain. Members of this family participate in diverse cellular processes that include membrane fusion, DNA replication, microtubule severing, and protein degradation. The protein encoded by this gene has a putative mitochondrial targeting sequence and has been proposed to function in maintenance of mitochondrial function and integrity during mouse spermatogenesis. Allelic variants in this gene have been associated with epilepsy, hearing loss, and cognitive disability syndrome. Alternative splicing results in multiple transcript variants.

Synonyms

Rho-related GTP-binding protein Rho6; Rho family GTPase 1; Rnd1; RND1; RHO6

Gene Name

SPATA5

Gene ID

166378

UniProt

Q8NB90

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SPATA5 recombinant protein (Position: E115-Q853) .

Clonality

Polyclonal

Tissue Specificity

Mostly expressed in brain and liver.

Applications

WB, Flow Cytometry, ELISA

Field of Research

Actin Assembly, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, G Protein Signaling, Microfilaments, Ras Family, Signal Transduction, Signaling Pathway, Small G Proteins

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Lacks intrinsic GTPase activity. Has a low affinity for GDP, and constitutively binds GTP. Controls rearrangements of the actin cytoskeleton. Induces the Rac-dependent neuritic process formation in part by disruption of the cortical actin filaments. Causes the formation of many neuritic processes from the cell body with disruption of the cortical actin filaments. .

References & Citations

1. Hartz, P. A. Personal Communication. Baltimore, Md. 4/19/2011. 2. Kurata, H., Terashima, H., Nakashima, M., Matsumura, W., Ohno, K., Saito, Y., Maegaki, Y., Kubota, M., Nanba, E., Saitsu, H., Matsumoto, N., Kato, M. Characterization of SPATA5-related encephalopathy in early childhood. Clin. Genet. 90: 437-444, 2016. 3. Liu, Y., Black, J., Kisiel, N., Kulesz-Martin, M. F. SPAF, a new AAA-protein specific to early spermatogenesis and malignant conversion. Oncogene 19: 1579-1588, 2000.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

26056 MW

Observed Molecular Weight

98 kDa

Applications Notes

6

Gene Name Synonym

Rho-related GTP-binding protein Rho6

Subcellular Location

Cell membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasm, cytoskeleton .

Protein Name

AP-2 complex subunit mu

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.