Anti-SPATA7 Antibody Picoband®

About this product

Boster Bio Anti-SPATA7 Antibody Picoband® catalog # A08310-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A08310-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Spermatogenesis-associated protein 7 is a protein that in humans is encoded by the SPATA7 gene. This gene, originally isolated from testis, is also expressed in retina. Mutations in this gene are associated with Leber congenital amaurosis and juvenile retinitis pigmentosa. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Synonyms

Histone-lysine N-methyltransferase SETDB2; Chronic lymphocytic leukemia deletion region gene 8 protein; Lysine N-methyltransferase 1F; SET domain bifurcated 2; SETDB2; C13orf4; CLLD8; KMT1F

Gene Name

SPATA7

Gene ID

55812

UniProt

Q9P0W8

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SPATA7 recombinant protein (Position: R5-V599) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed in reproductive organs, such as testis, ovary and prostate.

Applications

WB, Flow Cytometry, ELISA

Field of Research

Immune System Diseases, Immunology, Protein Trafficking, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3) . H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis.

References & Citations

1. Avila-Fernandez, A., Corton, M., Lopez-Molina, M. I., Martin-Garrido, E., Cantalapiedra, D., Fernandez-Sanchez, R., Blanco-Kelly, F., Riveiro-Alvarez, R., Tatu, S. D., Trujillo-Tiebas, M. J., Garcia-Sandoval, B., Ayuso, C., Cremers, F. P. M. Late onset retinitis pigmentosa. Ophthalmology 118: 2523-2524, 2011. 2. Eblimit, A., Agrawal, S. A., Thomas, K., Anastassov, I. A., Abulikemu, T., Moayedi, Y., Mardon, G., Chen, R. Conditional loss of Spata7 in photoreceptors causes progressive retinal degeneration in mice. Exp. Eye Res. 166: 120-130, 2018. Note: Erratum: Exp Eye Res. 171: 119, 2018. 3. Eblimit, A., Nguyen, T.-M. T., Chen, Y., Esteve-Rudd, J., Zhong, H., Letteboer, S., Van Reeuwijk, J., Simons, D. L., Ding, Q., Wu, K. M., Li, Y., Van Beersum, S., and 10 others. Spata7 is a retinal ciliopathy gene critical for correct RPGRIP1 localization and protein trafficking in the retina. Hum. Molec. Genet. 24: 1584-601, 2015.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

68 kDa

Applications Notes

6

Gene Name Synonym

SET domain bifurcated histone lysine methyltransferase 2

Subcellular Location

Nucleus. Chromosome.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.