Anti-SPATA18 Antibody Picoband®

About this product

Boster Bio Anti-SPATA18 Antibody Picoband® catalog # A09906-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A09906-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a p53-inducible protein that is able to induce lysosome-like organelles within mitochondria that eliminate oxidized mitochondrial proteins, thereby contributing to mitochondrial quality control. Dysregulation of mitochondrial quality control is associated with cancer and degenerative diseases. The encoded protein mediates accumulation of the lysosome-like mitochondrial organelles through interaction with B cell lymphoma 2 interacting protein 3 and B cell lymphoma 2 interacting protein 3 like at the outer mitochondrial membrane, which allows translocation of lysosomal proteins to the mitochondrial matrix from the cytosol. Alternative splicing results in multiple transcript variants.

Synonyms

Interleukin-36 alpha; FIL1 epsilon; Interleukin-1 epsilon; IL-1 epsilon; Interleukin-1 family member 6; IL-1F6; IL36A; FIL1E; IL1E; IL1F6

Gene Name

SPATA18

Gene ID

132671

UniProt

Q8TC71

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SPATA18 recombinant protein (Position: E27-R490) .

Clonality

Polyclonal

Tissue Specificity

Expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines. Predominantly expressed in skin keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Increased in lesional psoriasis skin.

Applications

ELISA, Flow Cytometry, IHC, WB

Field of Research

Cytokines, Immunology, Innate Immunity, Interleukins

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and inly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1, and the production of proinflammatory cytokines such as TNF-alpha, IL-8 and IL-6. In cultured monocytes upregulates expression of IL-1A, IL-1B and IL-6. In myeloid dendritic cells involved in cell maturation by upregulating surface expression of CD83, CD86 and HLA-DR. In monocyte-derived dendritic cells facilitates dendritic cell maturation and drives T-cell proliferation. May play a role in proinflammatory effects in the lung.

References & Citations

1. Bornstein, C., Brosh, R., Molchadsky, A., Madar, S., Kogan-Sakin, I., Goldstein, I., Chakravarti, D., Flores, E. R., Goldfinger, N., Sarig, R., Rotter, V. SPATA18, a spermatogenesis-associated gene, is a novel transcriptional target of p53 and p63. Molec. Cell. Biol. 31: 1679-1689, 2011. 2. Iida, H., Ichinose, J., Kaneko, T., Mori, T., Shibata, Y. Complementary DNA cloning of rat Spetex-1, a spermatid-expressing gene-1, encoding a 63 kDa cytoplasmic protein of elongate spermatids. Molec. Reprod. Dev. 68: 385-393, 2004. 3. Kaindl, A. M., Jakubiczka, S., Lucke, T., Bartsch, O., Weis, J., Stoltenburg-Didinger, G., Aksu, F., Oexle, K., Koehler, K., Huebner, A. Homozygous microdeletion of chromosome 4q11-q12 causes severe limb-girdle muscular dystrophy type 2E with joint hyperlaxity and contractures. (Abstract) Hum. Mutat. 26: 279-280, 2005. Note: Full article online.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

61 kDa

Applications Notes

6

Gene Name Synonym

Interleukin 36, alpha

Subcellular Location

Secreted.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.