Anti-SPRED2 Antibody Picoband®

About this product

Boster Bio Anti-SPRED2 Antibody Picoband® catalog # A06368-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A06368-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Sprouty-related, EVH1 domain-containing protein 2 is a protein that in humans is encoded by the SPRED2 gene. SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465) /SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade.

Synonyms

Rho-related GTP-binding protein Rho6; Rho family GTPase 1; Rnd1; RND1; RHO6

Gene Name

SPRED2

Gene ID

200734

UniProt

Q7Z698

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SPRED2 recombinant protein (Position: R40-R298) .

Clonality

Polyclonal

Tissue Specificity

Mostly expressed in brain and liver.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Actin Assembly, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, G Protein Signaling, Microfilaments, Ras Family, Signal Transduction, Signaling Pathway, Small G Proteins

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Lacks intrinsic GTPase activity. Has a low affinity for GDP, and constitutively binds GTP. Controls rearrangements of the actin cytoskeleton. Induces the Rac-dependent neuritic process formation in part by disruption of the cortical actin filaments. Causes the formation of many neuritic processes from the cell body with disruption of the cortical actin filaments. .

References & Citations

1. Bundschu, K., Knobeloch, K.-P., Ullrich, M., Schinke, T., Amling, M., Engelhardt, C. M., Renne, T., Walter, U., Schuh, K. Gene disruption of Spred-2 causes dwarfism. J. Biol. Chem. 280: 28572-28580, 2005. 2. Kato, R., Nonami, A., Taketomi, T., Wakioka, T., Kuroiwa, A., Matsuda, Y., Yoshimura, A. Molecular cloning of mammalian Spred-3 which suppresses tyrosine kinase-mediated Erk activation. Biochem. Biophys. Res. Commun. 302: 767-772, 2003. 3. Motta, M., Fasano, G., Gredy, S., Brinkmann, J., Bonnard, A. A., Simsek-Kiper, P. O., Gulec, E. Y., Essaddam, L., Utine, G. E., Guarnetti Prandi, I., Venditti, M., Pantaleoni, F., and 21 others. SPRED2 loss-of-function causes a recessive Noonan syndrome-like phenotype. Am. J. Hum. Genet. 108: 2112-2129, 2021.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

26056 MW

Observed Molecular Weight

53 kDa

Applications Notes

6

Gene Name Synonym

Rho-related GTP-binding protein Rho6

Subcellular Location

Cell membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasm, cytoskeleton .

Protein Name

AP-2 complex subunit mu

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.