Anti-SRP14 Antibody Picoband®

About this product

Boster Bio Anti-SRP14 Antibody Picoband® catalog # A07709-1. Tested in ELISA, Flow Cytometry, IP, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A07709-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.

Synonyms

Protein Hook homolog 3; h-hook3; Hhk3; HOOK3

Gene Name

SRP14

Gene ID

6727

UniProt

P37108

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SRP14 recombinant protein (Position: M1-D92) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed in chromaffin cells of the adrenal medulla (at protein level) . Detected in peripheral sympathetic ganglia (at protein level) . Found in some paracrine cells in stomach and duodenum (at protein level) .

Applications

ELISA, Flow Cytometry, IP, WB

Field of Research

Golgi Proteins, Protein Trafficking, Signal Transduction, Vesicle Transport

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Probably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking. Component of the FTS/Hook/FHIP complex (FHF complex) . The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex) . May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) .

References & Citations

1. Chang, D. Y., Nelson, B., Bilyeu, T., Hsu, K., Darlington, G. J., Maraia, R. J. A human Alu RNA-binding protein whose expression is associated with accumulation of small cytoplasmic Alu RNA. Molec. Cell. Biol. 14: 3949-3959, 1994. 2. Halic, M., Becker, T., Pool, M. R., Spahn, C. M. T., Grassucci, R. A., Frank, J., Beckmann, R. Structure of the signal recognition particle interacting with the elongation-arrested ribosome. Nature 427: 808-814, 2004. 3. Larsen, N., Samuelsson, T., Swieb, C. The Signal Recognition Particle Database (SRPDB) . Nucleic Acids Res. 26: 177-178, 1998.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

49229 MW

Observed Molecular Weight

18 kDa

Applications Notes

6

Gene Name Synonym

Hook microtubule tethering protein 3

Subcellular Location

Cytoskeleton. Golgi apparatus.

Protein Name

Chromaffin granule amine transporter

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.