Anti-SA1/STAG1 Antibody Picoband®

About this product

Boster Bio Anti-SA1/STAG1 Antibody Picoband® catalog # A06471-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A06471-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cohesin subunit SA-1 (SA1) is a protein that in humans is encoded by the STAG1 gene. This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase.

Synonyms

Mediator of RNA polymerase II transcription subunit 4; Activator-recruited cofactor 36 kDa component; ARC36; Mediator complex subunit 4; TRAP/SMCC/PC2 subunit p36 subunit; Vitamin D3 receptor-interacting protein complex 36 kDa component; DRIP36; MED4; ARC36; DRIP36; VDRIP; HSPC126

Gene Name

STAG1

Gene ID

10274

UniProt

Q8WVM7

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SA1/STAG1 recombinant protein (Position: Q748-L1224) .

Clonality

Polyclonal

Applications

WB, ICC, IF, Flow Cytometry, ELISA

Field of Research

Epigenetics and Nuclear Signaling, Mediator Complex, Pol II Transcription, Polymerase Associated Factors, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

References & Citations

1. Carramolino, L., Lee, B. C., Zaballos. A., Peled, A., Barthelemy, I., Shav-Tal, Y., Prieto, I., Carmi, P., Gothelf, Y., Gonzalez de Buitrago, G., Aracil, M., Marquez, G., Barbero, J. L., Zipori, D. SA-1, a nuclear protein encoded by one member of a novel gene family: molecular cloning and detection in hemopoietic organs. Gene 195: 151-159, 1997. Note: Erratum: Gene 206: 283 only, 1998. 2. Davidson, I. F., Bauer, B., Goetz, D., Tang, W., Wutz, G., Peters, J. M. DNA loop extrusion by human cohesin. Science 366: 1338-1345, 2019. 3. Lehalle, D., Mosca-Boidron, A.-L., Begtrup, A., Boute-Benejean, O., Charles, P., Cho, M. T., Clarkson, A., Devinsky, O., Duffourd, Y., Duplomb-Jego, L., Gerard, B., Jacquette, A., and 25 others. STAG1 mutations cause a novel cohesinopathy characterised by unspecific syndromic intellectual disability. J. Med. Genet. 54: 479-488, 2017.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

155 kDa

Applications Notes

6

Gene Name Synonym

Mediator complex subunit 4

Subcellular Location

Nucleus.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.