Anti-Rab4/RAB4A Antibody Picoband®

About this product

Boster Bio Anti-Rab4/RAB4A Antibody Picoband® catalog # A04643-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A04643-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene is a member of the largest group in the Ras superfamily of small GTPases, which regulate membrane trafficking. The encoded protein is associated with early endosomes and is involved in their sorting and recycling. The protein also plays a role in regulating the recycling of receptors from endosomes to the plasma membrane. Alternatively spliced transcript variants have been observed for this gene.

Synonyms

Solute carrier family 12 member 5; Electroneutral potassium-chloride cotransporter 2; K-Cl cotransporter 2; hKCC2; Neuronal K-Cl cotransporter; SLC12A5; KCC2; KIAA1176

Gene Name

RAB4A

Gene ID

5867

UniProt

P20338

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Rab4/RAB4A recombinant protein (Position: D110-C216) .

Clonality

Polyclonal

Tissue Specificity

Brain specific. Detected in neuronal cells.

Applications

WB, IHC, ELISA

Field of Research

Neuroscience, Neurotransmission, Potassium Channels, Receptors / Channels

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl- homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl- levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition. Involved in the regulation of dendritic spine formation and maturation.

References & Citations

1. Barbosa, M. D. F. S., Johnson, S. A., Achey, K., Gutierrez, M., Wakeland, E. K., Zerial, M., Kingsmore, S. F. The Rab protein family: genetic mapping of six Rab genes in the mouse. Genomics 30: 439-444, 1995. 2. Bielli, A., Thornqvist, P.-O., Hendrick, A. G., Finn, R., Fitzgerald, K., McCaffrey, M. W. The small GTPase Rab4A interacts with the central region of cytoplasmic dynein light intermediate chain-1. Biochem. Biophys. Res. Commun. 281: 1141-1153, 2001. 3. Goueli, B. S., Powell, M. B., Finger, E. C., Pfeffer, S. R. TBC1D16 is a Rab4A GTPase activating protein that regulates receptor recycling and EGF receptor signaling. Proc. Nat. Acad. Sci. 109: 15787-15792, 2012.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

24 kDa

Gene Name Synonym

Solute carrier family 12 member 5

Subcellular Location

Membrane. Multi-pass membrane protein.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.