Anti-WRS/WARS1 Antibody Picoband®

About this product

Boster Bio Anti-WRS/WARS1 Antibody Picoband® catalog # A02444-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02444-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA (trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene.

Synonyms

Cartilage oligomeric matrix protein; COMP; Thrombospondin-5; TSP5; COMP

Gene Name

WARS1

Gene ID

7453

UniProt

P23381

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human WRS/WARS1 recombinant protein (Position: E5-K369) .

Clonality

Polyclonal

Tissue Specificity

Abundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. Increased amounts are produced during late stages of osteoarthritis in the area adjacent to the main defect.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Signal Transduction, Cell Biology, Cell Cycle, Stem Cells, Cell Differentiation, Cytoskeleton/ECM, Extracellular Matrix, Structures, ECM Proteins, Mesenchymal Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP) . Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity) .

References & Citations

1. Borglum, A. D., Flint, T., Tommerup, N., Fleckner, J., Justesen, J., Kruse, T. A. Assignment of the human tryptophanyl-tRNA synthetase gene (WARS) to chromosome 14q32.2-q32.32. Cytogenet. Cell Genet. 73: 99-103, 1996. 2. Denney, R. M., Borgaonkar, D., Ruddle, F. H. Order of genes for NP and TRPRS on chromosome 14. Cytogenet. Cell Genet. 22: 493-497, 1978. 3. Francke, U., Denney, R. M., Ruddle, F. H. Intrachromosomal gene mapping in man: the gene for tryptophanyl-tRNA synthetase maps in region q21-qter of chromosome 14. Somat. Cell Genet. 3: 381-389, 1977.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

38779 MW

Observed Molecular Weight

55 kDa

Applications Notes

6

Gene Name Synonym

Cartilage oligomeric matrix protein

Subcellular Location

Secreted, extracellular space, extracellular matrix.

Protein Name

BAG family molecular chaperone regulator 1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.