Anti-RFX3 Antibody Picoband®

About this product

Boster Bio Anti-RFX3 Antibody Picoband® catalog # A07674-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A07674-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Transcription factor RFX3 is a protein that in humans is encoded by the RFX3 gene. This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. Multiple transcript variants encoding different isoforms have been described for this gene.

Synonyms

Cadherin-4; Retinal cadherin; R-CAD; R-cadherin; CDH4

Gene Name

RFX3

Gene ID

5991

UniProt

P48380

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human RFX3 recombinant protein (Position: Q283-V749) .

Clonality

Polyclonal

Tissue Specificity

Expressed mainly in brain but also found in other tissues.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Cadherins, Calcium Binding Proteins, Calcium Signaling, Cancer, Cell Adhesion, Cytoskeleton/ECM, Invasion/Microenvironment, Plasma Membrane, Signal Transduction, Signaling Pathway, Subcellular Markers, Tags & Cell Markers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May play an important role in retinal development.

References & Citations

1. Baas, D., Meiniel, A., Benadiba, C., Bonnafe, E., Meiniel, O., Reith, W., Durand, B. A deficiency in RFX3 causes hydrocephalus associated with abnormal differentiation of ependymal cells. Europ. J. Neurosci. 24: 1020-1030, 2006. 2. Bonnafe, E., Touka, M., AitLounis, A., Baas, D., Barras, E., Ucla, C., Moreau, A., Flamant, F., Dubruille, R., Couble, P., Collignon, J., Durand, B., Reith, W. The transcription factor RFX3 s nodal cilium development and left-right asymmetry specification. Molec. Cell. Biol. 24: 4417-4427, 2004. 3. Elkon, R., Milon, B., Morrison, L., Shah, M., Vijayakumar, S., Racherla, M., Leitch, C. C., Silipino, L., Hadi, S., Weiss-Gayet, M., Barras, E., Schmid, C. D., and 12 others. RFX transcription factors are essential for hearing in mice. Nature Commun. 6: 8549, 2015. Note: Electronic Article.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

157578 MW

Observed Molecular Weight

95 kDa

Applications Notes

6

Gene Name Synonym

Cadherin 4

Subcellular Location

Cell membrane. Single-pass type I membrane protein.

Protein Name

BAG family molecular chaperone regulator 5

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.