Anti-RAP1GAP Antibody Picoband®

About this product

Boster Bio Anti-RAP1GAP Antibody Picoband® catalog # A03561-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03561-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Rap1 GTPase-activating protein 1 is an enzyme that in humans is encoded by the RAP1GAP gene. This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene, RAP1GAP, promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins, guanine nucleotide exchange factors (GEFs), act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general, ras subfamily proteins, such as RAP1, play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation, adhesion, differentiation, and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins.

Synonyms

Non-homologous end-joining factor 1; Protein cernunnos; XRCC4-like factor; NHEJ1; XLF

Gene Name

RAP1GAP

Gene ID

5909

UniProt

P47736

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human RAP1GAP recombinant protein (Position: D11-L661) .

Clonality

Polyclonal

Tissue Specificity

Widely expressed.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Cell Biology, Cell Type Markers, Developmental Biology, Reproduction, Tags & Cell Markers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V (D) J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary. Binds DNA in a length-dependent manner.

References & Citations

1. Daumke, O., Weyand, M., Chakrabarti, P. P., Vetter, I. R., Wittinghofer, A. The GTPase-activating protein Rap1GAP uses a catalytic asparagine. Nature 429: 197-201, 2004. 2. Rubinfeld, B., Munemitsu, S., Clark, R., Conroy, L., Watt, K., Crosier, W. J., McCormick, F., Polakis, P. Molecular cloning of a GTPase activating protein specific for the Krev-1 protein p21-rap1. Cell 65: 1033-1042, 1991. 3. Weiss, J., Rubinfeld, B., Polakis, P. G., McCormick, F., Cavenee, W. K., Arden, K. C. The RAP1GA1 locus for human Rap1-GTPase activating protein 1 maps to chromosome 1p36.1-p35. Cytogenet. Cell Genet. 66: 18-21, 1994.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

75187 MW

Observed Molecular Weight

95 kDa

Applications Notes

6

Gene Name Synonym

Non-homologous end joining factor 1

Subcellular Location

Nucleus.

Protein Name

Segment polarity protein dishevelled homolog DVL-1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.