Anti-EZH1 Antibody Picoband®

About this product

Boster Bio Anti-EZH1 Antibody Picoband® catalog # A02494-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02494-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Histone-lysine N-methyltransferase EZH1 is an enzyme that in humans is encoded by the EZH1 gene. EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity.

Synonyms

Heat shock protein beta-8; HspB8; Alpha-crystallin C chain; E2-induced gene 1 protein; Protein kinase H11; Small stress protein-like protein HSP22; HSPB8; CRYAC, E2IG1, HSP22; PP1629

Gene Name

EZH1

Gene ID

2145

UniProt

Q92800

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human EZH1 recombinant protein (Position: R27-E485) .

Clonality

Polyclonal

Tissue Specificity

Predominantly expressed in skeletal muscle and heart. .

Applications

WB, ICC, IF, Flow Cytometry, ELISA

Field of Research

Chaperones, Heat Shock Proteins, Neurodegenerative Disease, Neurology Process, Neuroscience, Protein Trafficking, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Displays temperature-dependent chaperone activity.

References & Citations

1. Abel, K. J., Brody, L. C., Valdes, J. M., Erdos, M. R., McKinley, D. R., Castilla, L. H., Merajver, S. D., Couch, F. J., Friedman, L. S., Ostermeyer, E. A., Lynch, E. D., King, M.-C., Welcsh, P. L., Osborne-Lawrence, S., Spillman, M., Bowcock, A. M., Collins, F. S., Weber, B. L. Characterization of EZH1, a human homolog of Drosophila enhancer of zeste near BRCA1. Genomics 37: 161-171, 1996. 2. Laible, G., Haynes, A. R., Lebersorger, A., O'Carroll, D., Mattei, M.-G., Denny, P., Brown, S. D. M., Jenuwein, T. The murine polycomb-group genes Ezh1 and Ezh2 map close to Hox gene clusters on mouse chromosomes 11 and 6. Mammalian Genome 10: 311-314, 1999. 3. Maier, V. K., Feeney, C. M., Taylor, J. E., Creech, A. L., Qiao, J. W., Szanto, A., Das, P. P., Chevrier, N., Cifuentes-Rojas, C., Orkin, S. H., Carr, S. A., Jaffe, J. D., Mertins, P., Lee, J. T. Functional proteomic analysis of repressive histone methyltransferase complexes reveals ZNF518B as a G9A regulator. Molec. Cell. Proteomics 14: 1435-1446, 2015.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

21604 MW

Observed Molecular Weight

110 kDa, 60 kDa

Applications Notes

6

Gene Name Synonym

Heat shock protein beta-8

Subcellular Location

Cytoplasm . Nucleus . Translocates to nuclear foci during heat shock.

Protein Name

Heat shock protein beta-8

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.