Anti-Pirh2/RCHY1 Antibody Picoband®

About this product

Boster Bio Anti-Pirh2/RCHY1 Antibody Picoband® catalog # A04533-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A04533-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

RING finger and CHY zinc finger domain-containing protein 1 is a protein that in humans is encoded by the RCHY1 gene. The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Synonyms

Ras-related protein Rab-11B; GTP-binding protein YPT3; RAB11B; YPT3

Gene Name

RCHY1

Gene ID

25898

UniProt

Q96PM5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Pirh2/RCHY1 recombinant protein (Position: Q17-Q250) .

Clonality

Polyclonal

Tissue Specificity

Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.

Applications

WB, Flow Cytometry, ELISA

Field of Research

Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors ly responsible for vesicle formation, movement, tethering and fusion. That Rab plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis.

References & Citations

1. Fedorova, O. , Gudovich, A. , Daks, A. , Baidyuk, E. , & Barlev, N. A. . (2021) . Regulation of autophagy flux by e3 ubiquitin ligase pirh2 in lung cancer. Biochemical and Biophysical Research Communications, 563, 119-125. 2. Yifeng, Bao, Xue, Wu, Debin, & Yuan, et al. (2017) . High expression of pirh2 is associated with poor prognosis in glioma. Cellular and molecular neurobiology. 3. Yang, ShuyunChen, YingyingSun, FulanNi, QichaoWang, HuaHuang, YeqingZhang, ChunhuiLiu, KunWang, ShuoQiu, JianXu, ZhenHua, LuHe, Zhixian. (2016) . Downregulated pirh2 can decrease the proliferation of breast cancer cells. Archives of medical research, 47 (3) .

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

21 kDa

Applications Notes

6

Gene Name Synonym

RAB11B, member RAS oncogene family

Subcellular Location

Recycling endosome membrane, Lipid-anchor, Synaptic vesicle membrane, Phagosome membrane.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.