Anti-CYP27B1 Antibody Picoband®

About this product

Boster Bio Anti-CYP27B1 Antibody Picoband® catalog # A01370. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01370

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CYP27B1 belongs to the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.

Synonyms

Pleiotrophin; PTN; Heparin-binding brain mitogen; HBBM; Heparin-binding growth factor 8; HBGF-8; Heparin-binding growth-associated molecule; HB-GAM; Heparin-binding neurite outgrowth-promoting factor 1; HBNF-1; Osteoblast-specific factor 1; OSF-1; PTN; HBNF1; NEGF1

Gene Name

CYP27B1

Gene ID

1594

UniProt

O15528

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CYP27B1 recombinant protein (Position: R252-R508) .

Clonality

Polyclonal

Tissue Specificity

Osteoblast and brain.

Applications

WB, ELISA

Field of Research

Cancer, Cytokines, Cytoskeleton/ECM, Extracellular Matrix, Growth and Development, Growth Factors, Immunology, Innate Immunity, Neurology Process, Neuroscience, Signal Transduction, Structures

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells (PubMed:1768439, PubMed:1733956) . Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation (PubMed:11278720) . Binds to cell-surface target proteins via their chondroitin sulfate groups (PubMed:26896299) . Down-regulates PTPRZ1 activity (PubMed:16814777) .

References & Citations

1. Entrez Gene: cytochrome P450. 2. Monkawa T, Yoshida T, Wakino S, Shinki T, Anazawa H, Deluca HF et al. (Oct 1997) . Molecular cloning of cDNA and genomic DNA for human 25-hydroxyvitamin D3 1 alpha-hydroxylase. Biochemical and Biophysical Research Communications 239 (2) : 527–33. 3. Takeyama K, Kitanaka S, Sato T, Kobori M, Yanagisawa J, Kato S (Sep 1997) . 25-Hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis. Science (New York, N.Y.) 277 (5333) : 1827–30.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

57 kDa

Gene Name Synonym

Pleiotrophin

Subcellular Location

Secreted.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.