Anti-EIF4ENIF1 Antibody Picoband®

About this product

Boster Bio Anti-EIF4ENIF1 Antibody Picoband® catalog # A05520-2. Tested in ELISA, Flow Cytometry, ICC/IF, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05520-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Eukaryotic translation initiation factor 4E transporter is a protein that in humans is encoded by the EIF4ENIF1 gene. The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms

Spermine oxidase; Polyamine oxidase 1; PAO-1; PAOh1; Smox; Smo

Gene Name

EIF4ENIF1

Gene ID

56478

UniProt

Q9NRA8

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human EIF4ENIF1 recombinant protein (Position: D13-Q985) .

Clonality

Polyclonal

Tissue Specificity

Widely expressed. Isoform 1 and isoform 2 are expressed at higher level in brain and skeletal muscle. Isoform 7 is found in brain and spleen, isoform 10 is widely expressed but found at lower level in heart, kidney, liver and lung.

Applications

WB, ICC/IF, Flow Cytometry, ELISA

Field of Research

Chemokines, Immunology, Innate Immunity

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Flavoenzyme which catalyzes the oxidation of spermine to spermidine. Can also use N1-acetylspermine and spermidine as substrates, with different affinity depending on the isoform (isozyme) and on the experimental conditions. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. May contribute to beta-alanine production via aldehyde dehydrogenase conversion of 3-amino-propanal.

References & Citations

1. Dostie, J., Ferraiuolo, M., Pause, A., Adam, S. A., Sonenberg, N. A novel shuttling protein, 4E-T, mediates the nuclear import of the mRNA 5-prime cap-binding protein, eIF4E. EMBO J. 19: 3142-3156, 2000.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

48608 MW

Observed Molecular Weight

140 kDa

Applications Notes

6

Gene Name Synonym

Spermine oxidase

Subcellular Location

Cytoplasm. Nucleus. Cytoplasm.

Protein Name

Serine protease HTRA3

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.