Anti-LYPLA3/PLA2G15 Antibody Picoband®

About this product

Boster Bio Anti-LYPLA3/PLA2G15 Antibody Picoband® catalog # A08649. Tested in ELISA, , IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A08649

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

LYPLA3, also known as Group XV phospholipase A2, is an enzyme that in humans is encoded by the PLA2G15 gene. Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene hydrolyzes lysophosphatidylcholine to glycerophosphorylcholine and a free fatty acid. This enzyme is present in the plasma and thought to be associated with high-density lipoprotein. A later paper contradicts the function of this gene. It demonstrates that this gene encodes a lysosomal enzyme instead of a lysophospholipase and has both calcium-independent phospholipase A2 and transacylase activities.

Synonyms

Annexin A8 ; Annexin VIII ; Annexin-8 ; Vascular anticoagulant-beta ; VAC-beta ; ANXA8 ; ANX8

Gene Name

PLA2G15

Gene ID

23659

UniProt

Q8NCC3

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human LYPLA3/PLA2G15 recombinant protein (Position: D92-Q372) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed.

Applications

WB, ICC, IF, ELISA

Field of Research

Signal Transduction, Cardiovascular, Cytoskeleton, Cytoskeleton/ECM, Actin Binding Proteins, Actin, etc., Microfilaments, Cell Adhesion

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

This protein is an anticoagulant protein that acts as an in inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

References & Citations

1. Abe, A., Poucher, H. K., Hiraoka, M., Shayman, J. A. Induction of lysosomal phospholipase A2 through the retinoid X receptor in THP-1 cells. J. Lipid Res. 45: 667-673, 2004. 2. Hiraoka, M., Abe, A., Shayman, J. A. Cloning and characterization of a lysosomal phospholipase A2, 1-O-acylceramide synthase. J. Biol. Chem. 277: 10090-10099, 2002. 3. Taniyama, Y., Shibata, S., Kita, S., Horikoshi, K., Fuse, H., Shirafuji, H., Sumino, Y., Fujino, M. Cloning and expression of a novel lysophospholipase which structurally resembles lecithin cholesterol acyltransferase. Biochem. Biophys. Res. Commun. 257: 50-56, 1999.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

36881 MW

Observed Molecular Weight

42-57 kDa

Gene Name Synonym

Annexin A8

Subcellular Location

Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.

Protein Name

Annexin A8

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.