Anti-PRPF4 Antibody Picoband®

About this product

Boster Bio Anti-PRPF4 Antibody Picoband® catalog # A08225-2. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A08225-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

U4/U6 small nuclear ribonucleoprotein Prp4 is a protein that in humans is encoded by the PRPF4 gene. he protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Several transcript variants encoding different isoforms have been found for this gene.

Synonyms

Transforming growth factor beta activator LRRC32; Garpin; Glycoprotein A repetitions predominant; GARP; Leucine-rich repeat-containing protein 32; LRRC32; D11S833E

Gene Name

PRPF4

Gene ID

9128

UniProt

O43172

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PRPF4 recombinant protein (Position: R5-H461) .

Clonality

Polyclonal

Tissue Specificity

Heart, placenta, skeletal muscle, kidney, lung and pancreas.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Immune System Diseases, Immunology, Protein Trafficking, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space. Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta. Able to outcompete LTBP1 for binding to LAP regulatory chain of TGF-beta. Controls activation of TGF-beta-1 (TGFB1) on the surface of activated regulatory T-cells (Tregs) . Required for epithelial fusion during palate development by regulating activation of TGF-beta-3 (TGFB3) (By similarity) .

References & Citations

1. Chen, X., Liu, Y., Sheng, X., Tam, P. O. S., Zhao, K., Chen, X., Rong, W., Liu, Y., Liu, X., Pan, X., Chen, L. J., Zhao, Q., Vollrath, D., Pang, C. P., Zhao, C. PRPF4 mutations cause autosomal dominant retinitis pigmentosa. Hum. Molec. Genet. 23: 2926-2939, 2014. 2. Gonzalez-Santos, J. M., Wang, A., Jones, J., Ushida, C., Liu, J., Hu, J. Central region of the human splicing factor Hprp3p interacts with Hprp4p. J. Biol. Chem. 277: 23764-23772, 2002. 3. Heng, H. H. Q., Wang, A., Hu, J. Mapping of the human HPRP3 and HPRP4 genes encoding U4/U6-associated splicing factors to chromosomes 1q21.1 and 9q31-q33. Genomics 48: 273-275, 1998.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

55 kDa

Applications Notes

6

Gene Name Synonym

Leucine rich repeat containing 32

Subcellular Location

Cell membrane. Single-pass type I membrane protein. Cell surface.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.