Anti-3BP2/SH3BP2 Antibody Picoband®

About this product

Boster Bio Anti-3BP2/SH3BP2 Antibody Picoband® catalog # A04008-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A04008-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

SH3BP2 (SH3 domain-binding protein 2) is a protein that comes from a gene located on Chromosome 4. The protein encoded by this gene has an N-terminal pleckstrin homology (PH) domain, an SH3-binding proline-rich region, and a C-terminal SH2 domain. The protein binds to the SH3 domains of several proteins including the ABL1 and SYK protein tyrosine kinases , and functions as a cytoplasmic adaptor protein to positively regulate transcriptional activity in T, natural killer (NK), and basophilic cells. Mutations in this gene result in cherubism. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms

Ran-specific GTPase-activating protein; Ran-binding protein 1; RanBP1; RANBP1

Gene Name

SH3BP2

Gene ID

6452

UniProt

P78314

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human 3BP2/SH3BP2 recombinant protein (Position: R256-R561) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. In the kidney, it is widely expressed in tubules, but sparsely expressed in the glomerulus (PubMed:24676636) . Expression is significantly increased in renal biopsy specimens from idiopathic FSGS (PubMed:24676636) . Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.

Applications

ELISA, WB, Flow Cytometry

Field of Research

Cell Biology, Cell Cycle, Cell Division, DNA/RNA, Epigenetics and Nuclear Signaling, Nuclear Import/Export, Protein Trafficking, RNA Processing, Signal Transduction, Spindle

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Inhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane.

References & Citations

1. Bell, S. M., Shaw, M., Jou, Y.-S., Myers, R. M., Knowles, M. A. Identification and characterization of the human homologue of SH3BP2, an SH3 binding domain protein within a common region of deletion at 4p16.3 involved in bladder cancer. Genomics 44: 163-170, 1997. 2. Lo, B., Faiyaz-Ul-Haque, M., Kennedy, S., Aviv, R., Tsui, L.-C., Teebi, A. S. Novel mutation in the gene encoding c-Abl-binding protein SH3BP2 causes cherubism. Am. J. Med. Genet. 121A: 37-40, 2003. 3. Ueki, Y., Lin, C.-Y., Senoo, M., Ebihara, T., Agata, N., Onji, M., Saheki, Y., Kawai, T., Mukherjee, P. M., Reichenberger, E., Olsen, B. R. Increased myeloid cell responses to M-CSF and RANKL cause bone loss and inflammation in SH3BP2 'cherubism' mice. Cell 128: 71-83, 2007.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

23310 MW

Observed Molecular Weight

70 kDa

Applications Notes

6

Gene Name Synonym

Ran-specific GTPase-activating protein

Subcellular Location

Cytoskeleton. Nucleus. Cell cortex. Bleb.

Protein Name

Ran-specific GTPase-activating protein

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.