Anti-RFC4 Antibody Picoband®

About this product

Boster Bio Anti-RFC4 Antibody Picoband® catalog # A07702-1. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A07702-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Replication factor C subunit 4 is a protein that in humans is encoded by the RFC4 gene. The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) . RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.

Synonyms

Protein Hook homolog 3; h-hook3; Hhk3; HOOK3

Gene Name

RFC4

Gene ID

5984

UniProt

P35249

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human RFC4 recombinant protein (Position: E42-Q323) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed in chromaffin cells of the adrenal medulla (at protein level) . Detected in peripheral sympathetic ganglia (at protein level) . Found in some paracrine cells in stomach and duodenum (at protein level) .

Applications

WB, ICC, IF, Flow Cytometry, ELISA

Field of Research

Golgi Proteins, Protein Trafficking, Signal Transduction, Vesicle Transport

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Probably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking. Component of the FTS/Hook/FHIP complex (FHF complex) . The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex) . May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) .

References & Citations

1. Bowman, G. D., O'Donnell, M., Kuriyan, J. Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex. Nature 429: 724-730, 2004. 2. Chen, M., Pan, Z.-Q., Hurwitz, J. Studies of the cloned 37-kDa subunit of activator 1 (replication factor C) of HeLa cells. Proc. Nat. Acad. Sci. 89: 5211-5215, 1992. 3. Okumura, K., Nogami, M., Taguchi, H., Dean, F. B., Chen, M., Pan, Z.-Q., Hurwitz, J., Shiratori, A., Murakami, Y., Ozawa, K., Eki, T. Assignment of the 36.5-kDa (RFC5), 37-kDa (RFC4), 38-kDa (RFC3), and 40-kDa (RFC2) subunit genes of human replication factor C to chromosome bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23. Genomics 25: 274-278, 1995.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

49229 MW

Observed Molecular Weight

37 kDa

Applications Notes

6

Gene Name Synonym

Hook microtubule tethering protein 3

Subcellular Location

Cytoskeleton. Golgi apparatus.

Protein Name

Chromaffin granule amine transporter

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.