Anti-PFKFB1 Antibody Picoband®

About this product

Boster Bio Anti-PFKFB1 Antibody Picoband® catalog # A08398-2. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A08398-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 is an enzyme that in humans is encoded by the PFKFB1 gene. This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. Multiple alternatively spliced transcript variants have been found for this gene.

Synonyms

Disintegrin and metalloproteinase domain-containing protein 2; ADAM 2; Cancer/testis antigen 15; CT15; Fertilin subunit beta; PH-30; PH30; PH30-beta; ADAM2; FTNB

Gene Name

PFKFB1

Gene ID

5207

UniProt

P16118

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PFKFB1 recombinant protein (Position: R12-R186) .

Clonality

Polyclonal

Tissue Specificity

Expressed specifically in spermatogenic cells in the seminiferous cells. Not detected in fetal tissues.

Applications

WB, IHC, Flow Cytometry, ELISA

Field of Research

Cancer, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein.

References & Citations

1. Algaier, J., Uyeda, K. Molecular cloning, sequence analysis, and expression of a human liver cDNA coding for fructose-6,2-kinase:fructose-2,6-bisphosphatase. Biochem. Biophys. Res. Commun. 153: 328-333, 1988. 2. Batra, R. S., Hatchwell, E., Rider, S., Brown, R., Brown, G. K., Craig, I. W. Localization of human liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB1) within a YAC contig in Xp11.21. Genomics 40: 358-361, 1997. 3. Darville, M. I., Crepin, K. M., Vanderkerckhove, J., Van Damme, J., Octave, J. N., Rider, M. H., Marchand, M. J., Hue, L., Rousseau, G. G. Complete nucleotide sequence coding for rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase derived from a cDNA clone. FEBS Lett. 224: 317-321, 1987.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

82457 MW

Observed Molecular Weight

55 kDa

Applications Notes

6

Gene Name Synonym

Disintegrin and metalloproteinase domain-containing protein 2

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Name

Disintegrin and metalloproteinase domain-containing protein 2

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.