Anti-PPM1F Antibody Picoband®

About this product

Boster Bio Anti-PPM1F Antibody Picoband® catalog # A07776-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A07776-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein phosphatase 1F is an enzyme that in humans is encoded by the PPM1F gene. The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.

Synonyms

Protein SOX-15; Protein SOX-12; Protein SOX-20; SOX15; SOX12; SOX20; SOX26; SOX27

Gene Name

PPM1F

Gene ID

9647

UniProt

P49593

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PPM1F recombinant protein (Position: E17-S454) .

Clonality

Polyclonal

Tissue Specificity

Widely expressed in fetal and adult tissues examined, highest level found in fetal spinal cord and adult brain and testis.

Applications

WB, IHC, ICC/IF, IF, Flow Cytometry, ELISA

Field of Research

Golgi Proteins, Protein Trafficking, Signal Transduction, Vesicle Transport

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Binds to the 5'-AACAAT-3' sequence.

References & Citations

1. Gross, M. B. Personal Communication. Baltimore, Md. 4/30/2021. 2. Ishida, A., Sueyoshi, N., Kameshita, I. Functions and dysfunctions of Ca (2+) /calmodulin-dependent protein kinase phosphatase (CaMKP/PPM1F) and CaMKP-N/PPM1E. Arch. Biochem. Biophys. 640: 83-92, 2018. 3. Kim, P. R., Koon, Y. L., Lee, R. T. C., Azizan, F., Koh, D. H. Z., Chiam, K.-H., Koh, C.-G. Phosphatase POPX2 interferes with cell cycle by interacting with Chk1. Cell Cycle 19: 405-418, 2020.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

50 kDa

Observed Molecular Weight

50 kDa

Applications Notes

6

Gene Name Synonym

SRY-box 15

Subcellular Location

Nucleus.

Protein Name

Chromaffin granule amine transporter

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.