Anti-CYC1 Antibody Picoband®

About this product

Boster Bio Anti-CYC1 Antibody Picoband® catalog # A02958-2. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02958-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cytochrome c1, heme protein, mitochondrial (CYC1), also known as UQCR4, MC3DN6, Complex III subunit 4, Cytochrome b-c1 complex subunit 4, or Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit is a protein that in humans is encoded by the CYC1 gene. This gene encodes a subunit of the cytochrome bc1 complex, which plays an important role in the mitochondrial respiratory chain by transferring electrons from the Rieske iron-sulfur protein to cytochrome c. Mutations in this gene may cause mitochondrial complex III deficiency, nuclear type 6.

Synonyms

Oral-facial-digital syndrome 1 protein; Protein 71-7A; OFD1; CXorf5

Gene Name

CYC1

Gene ID

1537

UniProt

P08574

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CYC1 recombinant protein (Position: S85-K325) .

Clonality

Polyclonal

Tissue Specificity

Widely expressed. Expressed in 9 and 14 weeks old embryos in metanephric mesenchyme, oral mucosa, lung, heart, nasal and cranial cartilage, and brain. Expressed in metanephros, brain, tongue, and limb.

Applications

WB, ICC, IF, Flow Cytometry, ELISA

Field of Research

Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Component of the centrioles controlling mother and daughter centrioles length. Recruits to the centriole IFT88 and centriole distal appendage-specific proteins including CEP164. Involved in the biogenesis of the cilium, a centriole-associated function. The cilium is a cell surface projection found in many vertebrate cells required to transduce signals important for development and tissue homeostasis. Plays an important role in development by regulating Wnt signaling and the specification of the left-right axis. Only OFD1 localized at the centriolar satellites is removed by autophagy, which is an important step in the ciliogenesis regulation.

References & Citations

1. Duncan, A. M. V., Ozawa, T., Suzuki, H., Rozen, R. Assignment of the gene for the cytochrome c1 subunit of the mitochondrial cytochrome bc-1 complex (CYC1) to human chromosome 8q24.3. Genomics 19: 400-401, 1994. 2. Fukuyama, R., Minoshima, S., Nishikimi, M., Ozawa, T., Suzuki, H., Shimizu, N. Assignment of cytochrome c1 gene to chromosome 8q24 distal to MYC. (Abstract) Cytogenet. Cell Genet. 58: 1929 only, 1991. 3. Gaignard, P., Menezes, M., Schiff, M., Bayot, A., Rak, M., Ogier de Baulny, H., Su, C.-H., Gilleron, M., Lombes, A., Abida, H., Tzagoloff, A., Riley, L., and 11 others. Mutations in CYC1, encoding cytochrome c1 subunit of respiratory chain complex III, cause insulin-responsive hyperglycemia. Am. J. Hum. Genet. 93: 384-389, 2013.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

58026 MW

Observed Molecular Weight

33 kDa

Applications Notes

6

Gene Name Synonym

OFD1 centriole and centriolar satellite protein

Subcellular Location

Nucleus. Centriole. Cilium basal body. Centriolar satellite.

Protein Name

Oral-facial-digital syndrome 1 protein

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.