Anti-PRMT7 Antibody Picoband®

About this product

Boster Bio Anti-PRMT7 Antibody Picoband® catalog # A05485-2. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05485-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein arginine methyltransferase 7 is a protein that in humans is encoded by the PRMT7 gene. This gene encodes a member of the protein arginine N-methyltransferase family of proteins. The encoded enzyme transfers single methyl groups to arginine residues to generate monomethylarginines on histone proteins as well as other protein substrates. This enzyme plays a role in a wide range of biological processes, including neuronal differentiation, male germ line imprinting, small nuclear ribonucleoprotein biogenesis, and regulation of the Wnt signaling pathway. Mutations in this gene underlie multiple related syndromes in human patients characterized by intellectual disability, short stature and other features. The encoded protein may promote breast cancer cell invasion and metastasis in human patients.

Synonyms

Serine protease HTRA3;3.4.21.-; High-temperature requirement factor A3; Pregnancy-related serine protease; HTRA3; PRSP

Gene Name

PRMT7

Gene ID

54496

UniProt

Q9NVM4

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PRMT7 recombinant protein (Position: K121-D526) .

Clonality

Polyclonal

Tissue Specificity

Widely expressed, with highest levels in both adult and fetal heart, ovary, uterus placenta, and bladder. In the endometrium, expressed in epithelial glands and the stroma. Also present in leukocytes. Isoform 1 is predominant in heart and skeletal muscle, whereas isoform 2 is predominant in placenta and kidney. .

Applications

WB, IF, IHC, ICC, Flow Cytometry, ELISA

Field of Research

Alzheimer's Disease, Cell Biology, Metalloprotease, Neurodegenerative Disease, Neurology Process, Neuroscience, Proteolysis/Ubiquitin, Proteolytic Enzymes, Sensory System, Visual System

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Serine protease that cleaves beta-casein/CSN2 as well as several extracellular matrix (ECM) proteoglycans such as decorin/DCN, biglycan/BGN and fibronectin/FN1. Inhibits signaling mediated by TGF-beta family proteins possibly inly by degradation of these ECM proteoglycans (By similarity) . May act as a tumor suppressor. Negatively regulates, in vitro, trophoblast invasion during placental development and may be involved in the development of the placenta in vivo. May also have a role in ovarian development, granulosa cell differentiation and luteinization (PubMed:21321049, PubMed:22229724) . .

References & Citations

1. Akawi, N., McRae, J., Ansari, M., Balasubramanian, M., Blyth, M., Brady, A. F., Clayton, S., Cole, T., Deshpande, C., Fitzgerald, T. W., Foulds, N., Francis, R., and 30 others. Discovery of four recessive developmental disorders using probabilistic genotype and phenotype matching among 4,125 families. Nature Genet. 47: 1363-1369, 2015. 2. Birnbaum, R., Yosha-Orpaz, N., Yanoov-Sharav, M., Kidron, D., Gur, H., Yosovich, K., Lerman-Sagie, T., Malinger, G. Prenatal and postnatal presentation of PRMT7 related syndrome: expanding the phenotypic manifestations. Am. J. Med. Genet. 179A: 78-84, 2019. 3. Kernohan, K. D., McBride, A., Xi, Y., Martin, N., Schwartzentruber, J., Dyment, D. A., Majewski, J., Blaser, S. Care4Rare Canada Consortium, Boycott, K. M., Chitayat, D. Loss of the arginine methyltranserase (sic) PRMT7 causes syndromic intellectual disability with microcephaly and brachydactyly. Clin. Genet. 91: 708-716, 2017.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

48608 MW

Observed Molecular Weight

78 kDa

Applications Notes

6

Gene Name Synonym

Serine protease HTRA3

Subcellular Location

Secreted . Secretion increased during decidualization of endometrial stromal cells.

Protein Name

Serine protease HTRA3

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.