Anti-PUS7 Antibody Picoband®

About this product

Boster Bio Anti-PUS7 Antibody Picoband® catalog # A13434-1. Tested in ELISA, WB, IHC, ICC, IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A13434-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Enables enzyme binding activity and pseudouridine synthase activity. Involved in several processes, including pseudouridine synthesis; regulation of hematopoietic stem cell differentiation; and regulation of mesoderm development. Located in nucleus.

Synonyms

RNA-binding protein 47; RNA-binding motif protein 47; RBM47

Gene Name

PUS7

Gene ID

54517

UniProt

Q96PZ0

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human PUS7 recombinant protein (Position: D294-H541) .

Clonality

Polyclonal

Tissue Specificity

Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon. .

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Chromatin Binding Proteins, DNA/RNA Binding, Epigenetics and Nuclear Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Can bind to the surface of B-lymphoma cells, but not T- lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.

References & Citations

1. de Brouwer, A. P. M., Abou Jamra, R., Kortel, N., Soyris, C., Polla D. L., Safra, M., Zisso, A., Powell, C. A., Rebelo-Guiomar, P., Dinges, N., Morin, V., Stock, M., and 16 others. Variants in PUS7 cause intellectual disability with speech delay, microcephaly, short stature, and aggressive behavior. Am. J. Hum. Genet. 103: 1045-1052, 2018. 2. Han, S. T., Kim, A. C., Garcia, K., Schimmenti, L. A., Macnamara, E., Undiagnosed Diseases Network, Gahl, W. A., Malicdan, M. C., Tifft, C. J. PUS7 deficiency in human patients causes profound neurodevelopmental phenotype by dysregulating protein translation. Molec. Genet. Metab. 135: 221-229, 2022. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 3/10/2015.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

75 kDa

Observed Molecular Weight

75 kDa

Applications Notes

6

Gene Name Synonym

RNA-binding protein 47

Subcellular Location

Nucleus .

Protein Name

Follicular dendritic cell secreted peptide

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.