Anti-NIT2 Antibody Picoband®

About this product

Boster Bio Anti-NIT2 Antibody Picoband® catalog # A05891-3. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05891-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

NIT2 belongs to a branch of the nitrilase superfamily of enzymes that cleave carbon-nitrogen bonds. NIT2 functions as an omega-amidase and catalyzes hydrolysis of alpha-ketoglutaramate, forming alpha-ketoglutarate and ammonia. This reaction is also functionally coupled with a subset of transaminases that reaminate the keto acid analogs of some essential amino acids, most particularly methionine and phenylalanine.

Synonyms

Troponin T, fast skeletal muscle; TnTf; Beta-TnTF; Fast skeletal muscle troponin T; fTnT; TNNT3

Gene Name

NIT2

Gene ID

56954

UniProt

Q9NQR4

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NIT2 recombinant protein (Position: M1-K249) .

Clonality

Polyclonal

Tissue Specificity

In fetal and adult fast skeletal muscles, with a higher level expression in fetal than in adult muscle.

Applications

WB, IHC, Flow Cytometry, ELISA

Field of Research

Golgi Proteins, Organelles, Protein Trafficking, Signal Transduction, Subcellular Markers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

References & Citations

1. Asakura, M., Nakano, M., Hayashida, K., Fujii, H., Nakajima, M., Atsuda, K., Itoh, T., Fujiwara, R. Human nitrilase-like protein does not catalyze the hydrolysis of vildagliptin. Drug Metab. Pharmacokinet. 29: 463-469, 2014. 2. Chien, C.-H., Gao, Q.-Z., Cooper, A. J. L., Lyu, J.-H., Sheu, S.-Y. Structural insights into the catalytic active site and activity of human Nit2/omega-amidase: kinetic assay and molecular dynamics simulation. J. Biol. Chem. 287: 25715-25726, 2012. 3. Jaisson, S., Veiga-da-Cunha, M., Van Schaftingen, E. Molecular identification of omega-amidase, the enzyme that is functionally coupled with glutamine transaminases, as the putative tumor suppressor Nit2. Biochimie 91: 1066-1071, 2009.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

33 kDa

Applications Notes

6

Gene Name Synonym

Troponin T3, fast skeletal type

Subcellular Location

Troponin complex. Cytosol.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.