Anti-NUB1 Antibody Picoband®

About this product

Boster Bio Anti-NUB1 Antibody Picoband® catalog # A06089-2. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A06089-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

NEDD8 ultimate buster 1 is a protein that in humans is encoded by the NUB1 gene. This gene encodes a protein that functions as a negative regulator of NEDD8, a ubiquitin-like protein that conjugates with cullin family members in order to regulate vital biological events. The protein encoded by this gene regulates the NEDD8 conjugation system post-transcriptionally by recruiting NEDD8 and its conjugates to the proteasome for degradation. This protein interacts with the product of the AIPL1 gene, which is associated with Leber congenital amaurosis, an inherited retinopathy, and mutations in that gene can abolish interaction with this protein, which may contribute to the pathogenesis. This protein is also known to accumulate in Lewy bodies in Parkinson's disease and dementia with Lewy bodies, and in glial cytoplasmic inclusions in multiple system atrophy, with this abnormal accumulation being specific to alpha-synucleinopathy lesions. Alternative splicing of this gene results in multiple transcript variants.

Synonyms

RNA-binding protein Nova-1; Neuro-oncological ventral antigen 1; Onconeural ventral antigen 1; Paraneoplastic Ri antigen; Ventral neuron-specific protein 1; NOVA1

Gene Name

NUB1

Gene ID

51667

UniProt

Q9Y5A7

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NUB1 recombinant protein (Position: K190-D543) .

Clonality

Polyclonal

Tissue Specificity

Expressed in heart, placenta, skeletal muscle, brain, pancreas, lung, liver, but not kidney.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Cell Type Marker, Neuron Marker, Neuroscience

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

May regulate RNA splicing or metabolism in a specific subset of developing neurons.

References & Citations

1. Akey, D. T., Zhu, X., Dyer, M., Li, A., Sorensen, A., Blackshaw, S., Fukuda-Kamitani, T., Daiger, S. P., Craft, C. M., Kamitani, T., Sohocki, M. M. The inherited blindness associated protein AIPL1 interacts with the cell cycle regulator protein NUB1. Hum. Molec. Genet. 11: 2723-2733, 2002. Note: Erratum: Hum. Molec. Genet. 12: 451 only, 2003. 2. Kito, K., Yeh, E. T. H., Kamitani, T. NUB1, a NEDD8-interacting protein, is induced by interferon and down-regulates the NEDD8 expression. J. Biol. Chem. 276: 20603-20609, 2001.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

80420 MW

Observed Molecular Weight

75 kDa

Applications Notes

6

Gene Name Synonym

NOVA alternative splicing regulator 1

Subcellular Location

Nucleus.

Protein Name

MORC family CW-type zinc finger protein 3

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.