Anti-MNAT1 Antibody Picoband®

About this product

Boster Bio Anti-MNAT1 Antibody Picoband® catalog # A06523. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A06523

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CDK-activating kinase assembly factor MAT1 is an enzyme that in humans is encoded by the MNAT1 gene. The protein encoded by this gene, along with cyclin H and CDK7, forms the CDK-activating kinase (CAK) enzymatic complex. This complex activates several cyclin-associated kinases and can also associate with TFIIH to activate transcription by RNA polymerase II. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

Phospholipid-transporting ATPase IG (EC:7.6.2.1) ; ATPase IQ; ATPase class VI type 11C; P4-ATPase flippase complex alpha subunit ATP11C; ATP11C; ATPIG, ATPIQ

Gene Name

MNAT1

Gene ID

4331

UniProt

P51948

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MNAT1 recombinant protein (Position: M1-S309) .

Clonality

Polyclonal

Tissue Specificity

Widely expressed.

Applications

WB, ICC, IF, Flow Cytometry, ELISA

Field of Research

Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. In the cell membrane of erythrocytes, it is required to maintain phosphatidylserine (PS) in the inner leaflet preventing its exposure on the surface. This asymmetric distribution is critical for the survival of erythrocytes in circulation since externalized PS is a phagocytic signal for splenic macrophages. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. Required for B cell differentiation past the pro-B cell stage. Seems to mediate PS flipping in pro-B cells. May be involved in the transport of cholestatic bile acids.

References & Citations

1. Entrez Gene: MNAT1 menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis) . 2. Eki T, Okumura K, Abe M, Kagotani K, Taguchi H, Murakami Y, Pan ZQ, Hanaoka F (April 1998) . Mapping of the human genes encoding cyclin H (CCNH) and the CDK-activating kinase (CAK) assembly factor MAT1 (MNAT1) to chromosome bands 5q13.3-q14 and 14q23, respectively. Genomics 47 (1) : 115–20. 3. Talukder AH, Mishra SK, Mandal M, Balasenthil S, Mehta S, Sahin AA, Barnes CJ, Kumar R (March 2003) . MTA1 interacts with MAT1, a cyclin-dependent kinase-activating kinase complex ring finger factor, and regulates estrogen receptor transactivation functions. J. Biol. Chem. 278 (13) : 11676–85.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

27687 MW

Observed Molecular Weight

35 kDa

Applications Notes

6

Gene Name Synonym

ATPase phospholipid transporting 11C

Subcellular Location

Endoplasmic reticulum membrane. Cell membrane. Multi-pass membrane protein.

Protein Name

Phospholipid-transporting ATPase IG

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.