Anti-METAP2 Antibody Picoband®

About this product

Boster Bio Anti-METAP2 Antibody Picoband® catalog # A03648-2. Tested in ELISA, IHC, WB, applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03648-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Methionine aminopeptidase 2 is an enzyme that in humans is encoded by the METAP2 gene. The protein encoded by this gene is a member of the methionyl aminopeptidase family. The encoded protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent proteins. Increased expression of this gene is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. Inhibitors of this gene have also been shown to be effective for the treatment of obesity. A pseudogene of this gene is located on chromosome 2. Several transcript variants encoding different isoforms have been found for this gene.

Synonyms

DNA replication licensing factor MCM5; CDC46 homolog; P1-CDC46; MCM5; CDC46

Gene Name

METAP2

Gene ID

10988

UniProt

P50579

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human METAP2 recombinant protein (Position: E201-R474) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitous.

Applications

WB, IHC, ELISA

Field of Research

ER Proteins, Golgi Proteins, Protein Trafficking, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Interacts with MCMBP.

References & Citations

1. Datta, B., Chakrabarti, D., Roy, A. L., Gupta, N. K. Roles of a 67-kDa polypeptide in reversal of protein synthesis inhibition in heme-deficient reticulocyte lysate. Proc. Nat. Acad. Sci. 85: 3324-3328, 1988. 2. Griffith, E. C., Su, Z., Niwayama, S., Ramsay, C. A., Chang, Y- H., Liu, J. O. Molecular recognition of angiogenesis inhibitors fumagillin and ovalicin by methionine aminopeptidase 2. Proc. Nat. Acad. Sci. 95: 15183-15188, 1998. 3. Gross, M. B. Personal Communication. Baltimore, Md. 5/21/2014.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

28461 MW

Observed Molecular Weight

67 kDa

Gene Name Synonym

Minichromosome maintenance complex component 5

Subcellular Location

Nucleus. Cytosol.

Protein Name

Breast cancer metastasis-suppressor 1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.