Anti-MMS19 Antibody Picoband®

About this product

Boster Bio Anti-MMS19 Antibody Picoband® catalog # A06218-2. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A06218-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

MMS19 nucleotide excision repair protein homolog is a protein that in humans is encoded by the MMS19 gene. MMS19 (MMS19 nucleotide excision repair homolog), also known as MET18, is a 1,030 amino acid nuclear protein containing seven HEAT repeats that belongs to the MET18/MMS19 family. Via its interactions with TFIIH p80 and TFIIH p89 helicases, MMS19 plays a role in nucleotide excision repair (NER) and RNA polymerase II (Pol II) transcription. MMS19 may also function as a transcriptional coactivator of estrogen receptor. While ubiquitously expressed, highest levels of MMS19 have been found in testis. At least five distinct MMS19 protein isoforms exist, which are produced by alternative splicing events.

Synonyms

ELAV-like protein 2; ELAV-like neuronal protein 1; Hu-antigen B; HuB; Nervous system-specific RNA-binding protein Hel-N1; ELAVL2; HUB

Gene Name

MMS19

Gene ID

64210

UniProt

Q96T76

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MMS19 recombinant protein (Position: E239-R998) .

Clonality

Polyclonal

Tissue Specificity

Brain; neural-specific.

Applications

ELISA, IF, IHC, ICC, WB, Flow Cytometry

Field of Research

DNA/RNA, Epigenetics and Nuclear Signaling, RNA Processing

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Binds RNA. Seems to recognize a GAAA motif. Can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR.

References & Citations

1. Gari, K., Leon Ortiz, A. M., Borel, V., Flynn, H., Skehel, J. M., Boulton, S. J. MMS19 links cytoplasmic iron-sulfur cluster assembly to DNA metabolism. Science 337: 243-245, 2012. 2. Hatfield, M. D., Reis, A. M. C., Obeso, D., Cook, J. R., Thompson, D. M., Rao, M., Friedberg, E. C., Queimado, L. Identification of MMS19 domains with distinct functions in NER and transcription. DNA Repair 5: 914-924, 2006. 3. Ito, S., Tan, L. J., Andoh, D., Narita, T., Seki, M., Hirano, Y., Narita, K., Kuraoka, I., Hiraoka, Y., Tanaka, K. MMXD, a TFIIH-independent XPD-MMS19 protein complex involved in chromosome segregation. Molec. Cell 39: 632-640, 2010.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

49655 MW

Observed Molecular Weight

113 kDa

Applications Notes

6

Gene Name Synonym

ELAV like RNA binding protein 2

Subcellular Location

Cytoplasm. Nucleus. Cytoplasm.

Protein Name

AP-2 complex subunit mu

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.