Anti-EB1/MAPRE1 Antibody Picoband®

About this product

Boster Bio Anti-EB1/MAPRE1 Antibody Picoband® catalog # A02070-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02070-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Microtubule-associated protein RP/EB family member 1 is a protein that in humans is encoded by the MAPRE1 gene. The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family.

Synonyms

Deleted in azoospermia-like; DAZ homolog; DAZ-like autosomal; Deleted in azoospermia-like 1; SPGY-like-autosomal; DAZL; DAZH; DAZL1; DAZLA; SPGYLA

Gene Name

MAPRE1

Gene ID

22919

UniProt

Q15691

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human EB1/MAPRE1 recombinant protein (Position: A145-Y268) .

Clonality

Polyclonal

Applications

WB, Flow Cytometry, ELISA

Field of Research

Angiogenesis, Cancer, Cardiovascular, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

RNA-binding protein, which is essential for gametogenesis in both males and females. Plays a central role during spermatogenesis. Acts by binding to the 3'-UTR of mRNA, specifically recognizing GUU triplets, and thereby regulating the translation of key transcripts.

References & Citations

1. Berrueta, L., Kraeft, S.-K., Tirnauer, J. S., Schuyler, S. C., Chen, L. B., Hill, D. E., Pellman, D., Bierer, B. E. The adenomatous polyposis coli-binding protein EB1 is associated with cytoplasmic and spindle microtubules. Proc. Nat. Acad. Sci. 95: 10596-10601, 1998. 2. Berrueta, L., Tirnauer, J. S., Schuyler, S. C., Pellman, D., Bierer, B. E. The APC-associated protein EB1 associates with components of the dynactin complex and cytoplasmic dynein intermediate chain. Curr. Biol. 9: 425-428, 1999. 3. Jiang, K., Wang, J., Liu, J., Ward, T., Wordeman, L., Davidson, A., Wang, F, Yao, X. TIP150 interacts with and targets MCAK at the microtubule plus ends. EMBO Rep. 10: 857-865, 2009.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

35 kDa

Applications Notes

6

Gene Name Synonym

Deleted in azoospermia like

Subcellular Location

Nucleus. Cytoplasm.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.