Anti-hIST1/IST1 Antibody Picoband®

About this product

Boster Bio Anti-hIST1/IST1 Antibody Picoband® catalog # A05507-2. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05507-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

IST1 homolog is a protein that in humans is encoded by the KIAA0174 gene. This gene encodes a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT) . ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis. There is a pseudogene for this gene on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Synonyms

70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70 (S6K) alpha antibody, p70 (S6K) -alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody

Gene Name

IST1

Gene ID

9798

UniProt

P53990

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross reactivity with other proteins.

Immunogen

E.coli-derived human hIST1/IST1 recombinant protein (Position: E31-D331) . Human IST1 shares 94% and 93.1% amino acid (aa) sequence identity with mouse and rat IST1, respectively.

Clonality

Polyclonal

Tissue Specificity

Expressed in all tissues.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Signal Transduction, Signaling Pathway, Small G Proteins

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.

References & Citations

1. Agromayor, M., Carlton, J. G., Phelan, J. P., Matthews, D. R., Carlin, L. M., Ameer-Beg, S., Bowers, K., Martin-Serrano, J. Essential role of hIST1 in cytokinesis. Molec. Biol. Cell 20: 1374-1387, 2009. 2. Bajorek, M., Morita, E., Skalicky, J. J., Morham, S. G., Babst, M., Sundquist, W. I. Biochemical analyses of human IST1 and its function in cytokinesis. Molec. Biol. Cell 20: 1360-1373, 2009. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 6/23/2015.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

52588 MW

Observed Molecular Weight

40 kDa

Applications Notes

6

Gene Name Synonym

Serine/threonine-protein kinase AtPK1/AtPK6

Subcellular Location

Cytoplasm. Nucleus.

Protein Name

Serine/threonine-protein kinase AtPK1/AtPK6

Isotype

IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.