- Catalog number
- A01654-1
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- The Linker for activation of T cells, also known as linker of activated T cells or LAT, is a protein involved in the T-cell antigen receptor signal transduction pathway which in humans is encoded by the LAT gene. The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.
- Synonyms
- 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70 (S6K) alpha antibody, p70 (S6K) -alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody
- Gene Name
- LAT
- Gene ID
- 27040
- UniProt
- O43561
- Host
- Rabbit
- Reactivity
- Human
- Cross Reactivity
- No cross reactivity with other proteins.
- Immunogen
- E.coli-derived human LAT recombinant protein (Position: H28-E235) . Human LAT shares 61% and 59.3% amino acid (aa) sequence identity with mouse and rat LAT, respectively.
- Clonality
- Polyclonal
- Tissue Specificity
- Expressed in all tissues.
- Applications
- ELISA, WB, Flow Cytometry
- Field of Research
- Signal Transduction, Signaling Pathway, Small G Proteins
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Function
- Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.
- References & Citations
- 1. Aguado, E., Richelme, S., Nunez-Cruz, S., Miazek, A., Mura, A.-M., Richelme, M., Guo, X.-J., Sainty, D., He, H.-T., Malissen, B., Malissen, M. Induction of T helper type 2 immunity by a point mutation in the LAT adaptor. Science 296: 2036-2040, 2002. 2. Bacchelli, C., Moretti, F. A., Carmo, M., Adams, S., Stanescu, H. C., Pearce, K., Madkaikar, M., Gilmour, K. C., Nicholas, A. K., Woods, C. G., Kleta, R., Beales, P. L., Qasim, W., Gaspar, H. B. Mutations in linker for activation of T cells (LAT) lead to a novel form of severe combined immunodeficiency. J. Allergy Clin. Immun. 139: 634-642, 2017. 3. Hundt, M., Tabata, H., Jeon, M.-S., Hayashi, K., Tanaka, Y., Krishna, R., De Giorgio, L., Liu, Y.-C., Fukata, M., Altman, A. Impaired activation and localization of LAT in anergic T cells as a consequence of a selective palmitoylation defect. Immunity 24: 513-522, 2006.
- Storage Conditions
- At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
- Calculated Molecular Weight
- 52588 MW
- Observed Molecular Weight
- 37 kDa
- Applications Notes
- 6
- Gene Name Synonym
- Serine/threonine-protein kinase AtPK1/AtPK6
- Subcellular Location
- Cytoplasm. Nucleus.
- Protein Name
- Serine/threonine-protein kinase AtPK1/AtPK6
- Isotype
- IgG
- Contents
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
