Anti-LARG/ARHGEF12 Antibody Picoband®

About this product

Boster Bio Anti-LARG/ARHGEF12 Antibody Picoband® catalog # A06802-3. Tested in WB, IHC, ICC, IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A06802-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Rho guanine nucleotide exchange factor 12 is a protein that in humans is encoded by the ARHGEF12 gene. Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene.

Gene Name

ARHGEF12

Gene ID

23365

UniProt

Q9NZN5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human LARG/ARHGEF12 recombinant protein (Position: D10-E1426) . Human ARHGEF12 shares 89.9% amino acid (aa) sequence identity with mouse ARHGEF12.

Clonality

Polyclonal

Applications

ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

References & Citations

1. Goulimari, P., Knieling, H., Engel, U., Grosse, R. LARG and mDia1 link G-alpha-12/13 to cell polarity and microtubule dynamics. Molec. Biol. Cell 19: 30-40, 2008. 2. Kourlas, P. J., Strout, M. P., Becknell, B., Veronese, M. L., Croce, C. M., Theil, K. S., Krahe, R., Ruutu, T., Knuutila, S., Bloomfield, C. D., Caligiuri, M. A. Identification of a gene at 11q23 encoding a guanine nucleotide exchange factor: evidence for its fusion with MLL in acute myeloid leukemia. Proc. Nat. Acad. Sci. 97: 2145-2150, 2000. 3. Martz, M. K., Grabocka, E., Beeharry, N., Yen, T. J., Wedegaertner, P. B. Leukemia-associated RhoGEF (LARG) is a novel RhoGEF in cytokinesis and required for the proper completion of abscission. Molec. Biol. Cell 24: 2785-2794, 2013.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

220 kDa

Applications Notes

6

Isotype

IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.