Anti-MED7 Antibody Picoband®

About this product

Boster Bio Anti-MED7 Antibody Picoband® catalog # A09822. Tested in WB, ICC/IF, IP, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A09822

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mediator of RNA polymerase II transcription subunit 7 is an enzyme that in humans is encoded by the MED7 gene. The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene.

Gene Name

MED7

Gene ID

9443

UniProt

O43513

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human MED7 recombinant protein (Position: M1-P233) . Human MED7 shares 97% amino acid (aa) sequence identity with mouse MED7.

Clonality

Polyclonal

Applications

WB, ICC, IF, IP, ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

References & Citations

1. Myers, L. C., Gustafsson, c. M., Bushnell, D. A., Lui, M., Erdjument-Bromage, H., Tempst, P., Kornberg, R. D. The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. Genes Dev. 12: 45-54, 1998. 2. Ryu, S., Zhou, S., Ladurner, A. G., Tjian, R. The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1. Nature 397: 446-450, 1999. 3. Sato, S., Tomomori-Sato, C., Parmely, T. J., Florens, L., Zybailov, B., Swanson, S. K., Banks, C. A. S., Jin, J., Cai, Y., Washburn, M. P., Conaway, J. W., Conaway, R. C. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. Molec. Cell 14: 685-691, 2004.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

27 kDa

Observed Molecular Weight

27 kDa

Isotype

IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.